The metabolically stable protein kinase A inhibitor Rp-cAMPS (Cat. No. A 002) immobilized on agarose by an aminohexylamino spacer attached to position 8 of the ligand. Free Rp-cAMPS binds to the protein kinase A holoenzyme but prevents dissociation of catalytic and regulatory subunits. In addition it is not hydrolized by mammalian phosphodiesterases. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinase A (presumably as its holoenzyme), phosphodiesterases and others. This ligand is offered with a shorter spacer (Rp-8-AEA-cAMPS-Agarose, Cat. No. A 007) as well. Detailed technical information available. References: Margarucci et al., Mol. BioSyst., 7, 2311 - 2319 (2011); Bertinetti et al., BMC Chem Biol, 9 (2009).
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Appearance: Suspension in 30 mM Na2HPO4 buffer (pH 7).
Cat. No. | A 012 |
CAS number | [pending] |
Appearance | Suspension in 30 mM Na₂HPO₄ buffer (pH 7) |
Storage temperature | 4°C / 39°F |
cAMP modified at the exocyclic oxygen | equatorial (Rp-) |
Modifications | 8-cAMP |