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EtOH-NH-Agarose

Ethanolamine immobilized on agarose gel ( EtOH-NH-Agarose )
Control gel
Cat. No.: E 010
    
CAS No.: 
Shipping: ambient
Product Name Price (net) Qty
Cat. No.: E 010-06
Unit: 0.6 ml
$107.00
Cat. No.: E 010-25
Unit: 2.5 ml
$335.00
Cat. No.: E 010-60
Unit: 6 ml
$672.00

Agarose gel without nucleotide ligands, as negative control in affinity chromatography experiments. This agarose matrix is identical to the material used for synthesis of the affinity gels offered by BIOLOG. The reactive groups of the bead surface normally used to immobilize the functionalized nucleotide, have been deactivated with ethanolamine. References: Antl et al., Blood, 109, 552 - 559 (2007); Hammerschmidt et al., PLoS One 7(7): e39848 (2012).

Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.

 

Appearance: Suspension in 30 mM Na2HPO4 buffer (pH 7).

Cat. No. E 010
Appearance Suspension in 30 mM Na₂HPO₄ buffer (pH 7)
Storage temperature 4°C / 39°F
  • 1. Donaldson L.Meier S., Methods Mol. Biol., 1016, 155 - 173 (2013), "An Affinity Pull-Down Approach to Identify the Plant Cyclic Nucleotide Interactome"
  • 2. Hammerschmidt A., Chatterji B., Zeiser J., Schröder A., Genieser H.-G., Pich A., Kaever V., Schwede F., Wolter S.Seifert R., PLoS ONE, 7, 39848 - 0 (2012), "Binding of Regulatory Subunits of cyclic AMP-dependent Protein Kinase to cyclic CMP Agarose"
  • 3. Antl M., von Brühl M.-L., Eiglsperger C., Werner M., Konrad I., Kocher T., Wilm M., Hofmann F., Massberg S.Schlossmann J., Blood, 109, 552 - 559 (2007), "IRAG Mediates NO/cGMP-dependent Inhibition of Platelet Aggregation and Thrombus Formation"
  • 4. Deng W.Baker D. A., Mol. Microbiol., 44, 1141 - 1151 (2002), "A Novel Cyclic GMP-dependent Protein Kinase is Expressed in the Ring Stage of the Plasmodium Falciparum Life Cycle"