Alternative structural projections:
The protein kinase A activator Sp-cAMPS (Cat. No. A 003) immobilized on agarose by an aminohexylamino spacer attached to position 8 of the ligand. Free Sp-cAMPS is only very slowly metabolized by mammalian phosphodiesterases and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase A compared to cAMP results in milder desorption conditions in affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This ligand is offered with a shorter spacer (Sp-8-AEA-cAMPS-Agarose, Cat. No. A 008) as well. Detailed technical information available. Reference: Bertinetti et al., BMC Chem Biol, 9 (2009).
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Appearance: Suspension in 30 mM Na2HPO4 buffer (pH 7).
| Cat. No. | A 013 |
| CAS number | [pending] |
| Appearance | Suspension in 30 mM Na₂HPO₄ buffer (pH 7) |
| Storage temperature | 4°C / 39°F |
| cAMP modified at the exocyclic oxygen | axial (Sp-) |
| Modifications | 8-cAMP |
- 1. Hanke S. E., Bertinetti D., Badel A., Schweinsberg S., Genieser H.-G.Herberg F. W., N. Biotechnol., 28, 294 - 301 (2011), "Cyclic Nucleotides as Affinity Tools: Phosphorothioate cAMP Analogues Adress Specific PKA Subproteomes"
- 2. Bertinetti D., Schweinsberg S., Hanke S. E., Schwede F., Bertinetti O., Drewianka S., Genieser H.-G.Herberg F. W., BMC Chem Biol, 9, 0 - 0 (2009), "Chemical tools selectively target components of the PKA system"
