The protein kinase A inhibitor Rp-cAMPS (Cat. No. A 002) immobilized on agarose by an aminoethylcarbamoyl spacer attached to the ribose 2'-position of the ligand. Free Rp-cAMPS is not metabolized by mammalian phosphodiesterases and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase A compared to cAMP results in milder desorption conditions during affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins, especially those which tolerate modification of the 2'-ribose hydroxyl group such as some phosphodiesterases. This type of gel is offered with a longer spacer (Rp-2'-AHC-cAMPS-Agarose, Cat. No. A 049) as well. Detailed technical information available.
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Appearance: Suspension in 30 mM Na2HPO4 buffer (pH 7).
Cat. No. | A 126 |
CAS number | [pending] |
Appearance | Suspension in 30 mM Na₂HPO₄ buffer (pH 7) |
Storage temperature | 4°C / 39°F |
cAMP modified at the exocyclic oxygen | equatorial (Rp-) |
Modifications | 2'-cAMP |