For other salt forms please inquire. The azide-containing 5-N₃Ac-AA-dUTP is an analogue of dUTP and a suitable molecular tool for the copper(I)-catalysed Huisgen azide-alkyne [3+2] cycloaddition (CuAAC) or copper(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) with strained alkynes, respectively. Both reactions have attracted particular interest for the introduction of reporter groups and for the labelling of various biomolecules like TTP-binding proteins by the so-called click-chemistry technique. 5-Azide-functionalised pyrimidine nucleotides can replace their natural counterpart TTP in polymerase reactions, leading to the generation of a modified and functionalized DNA strand that can subsequently be further reacted and modified using click chemistry methods. References for CuAAC and SPAAC click chemistry: Dommerholt et al., Top. Curr. Chem., 374:16 (2016); Presolski et al., Curr. Protoc. Chem. Biol., 3, 153 - 162 (2011); Hong et al., Angew. Chem. Int. Ed. Engl., 48, 9879 - 9883 (2009)
Vial containing 250 µl of 1 mM aqueous solution of pH 7.6
Cat. No. | A 358 |
CAS number | [pending] |
Purity | > 95% HPLC |
Salt form | Sodium |
Appearance | 1 mM aqueous solution of pH 7.6 |
Storage temperature | -70°C / -94°F |
Light-sensitive | Yes |
Molecular formula | C₁₄H₂₁N₆O₁₅P₃ (free acid) |
Molecular weight [g/mol] | 606.3 (free acid) |
Absorption max [nm] | 290 |
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] | 8100 |
UV pH value | 7 |
Solvent for UV data | Water |