0 Items - $0.00
Categories
A to Z

Alternative structural projections:

8-AHA-cAMP-Agarose

8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphate; immobilized on agarose gel ( 8-AHA-cAMP-Agarose )
cyclic AMP immobilized on agarose gel 
Cat. No.: A 028
    
CAS No.: [pending]
Shipping: ambient
Product Name Price (net) Qty
Cat. No.: A 028-06
Unit: 0.6 ml
$99.00
Cat. No.: A 028-25
Unit: 2.5 ml
$310.00
Cat. No.: A 028-60
Unit: 6 ml
$624.00
The second messenger cyclic AMP immobilized on agarose by an aminohexylamino spacer attached to position 8 of the ligand. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This ligand is also offered with a shorter spacer (8-AEA-cAMP-Agarose, Cat. No. A 020) and as PDE-resistant version (Sp-8-AHA-cAMPS-Agarose, Cat. No. A 013). Detailed technical information available. References: Dills et al., Biochemistry, 15, 3724 - 3731 (1976); Margarucci et al., Mol. BioSyst. 7, 2311 - 2319 (2011).  Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Cat. No. A 028
CAS number [pending]
Storage temperature 4°C / 39°F
Modifications 8-cAMP
  • 1. Hanke S. E., Bertinetti D., Badel A., Schweinsberg S., Genieser H.-G.Herberg F. W., N. Biotechnol., 28, 294 - 301 (2011), "Cyclic Nucleotides as Affinity Tools: Phosphorothioate cAMP Analogues Adress Specific PKA Subproteomes"
  • 2. Margarucci L., Roest M., Preisinger C., Bleijerveld O. B., van Holten T. C., Heck A. J. R.Scholten A., Mol. Biosyst., 7, 2311 - 2319 (2011), "Collagen Stimulation of Platelets Induces a Rapid Spatial Response of cAMP and cGMP Signaling Scaffolds"
  • 3. Bertinetti D., Schweinsberg S., Hanke S. E., Schwede F., Bertinetti O., Drewianka S., Genieser H.-G.Herberg F. W., BMC Chem Biol, 9, 0 - 0 (2009), "Chemical tools selectively target components of the PKA system"
  • 4. Stokka A. J., Gesellchen F., Carlson C. R., Scott J. D., Herberg F. W.Tasken K., Biochem. J., 400, 493 - 499 (2006), "Characterization of A-kinase-anchoring Disruptors Using a Solution-based Assay"
  • 5. Hahnefeld C., Diploma work Bochum Germany, 0, 0 - 0 (2000), "Untersuchungen zur Nukleotidbindung von cAMP-abhängigen Proteinkinasen"
  • 6. Pöhler D., Butt E., Meißner J., Müller S., Walter U., Lohmann S. M.Jarchau T., FEBS Lett., 374, 419 - 425 (1995), "Expression, Purification, and Characterization of the cGMP-dependent Protein Kinases Iß and II Using the Baculovirus System"
  • 7. Johnson R. L., Van Haastert P. J. M., Kimmel A. R., Saxe III C. L., Jastorff B.Devreotes P. N., J. Biol. Chem., 267, 4600 - 4607 (1992), "The Cyclic Nucleotide Specificity of Three cAMP Receptors in Dictyostelium"
  • 8. Theibert A., Palmisano M., Jastorff B.Devreotes P. N., Develop. Biol., 114, 529 - 533 (1986), "The Specificity of the cAMP Receptor Mediating Activation of Adenylate Cyclase in Dictyostelium Discoideum"
  • 9. Brandt C., Jastorff B.Hildebrandt A., Roux's Arch. Dev. Biol., 195, 276 - 280 (1986), "Sporulation Delay by Stable cAMP Analogs in the Slime Mould Physarum Polycephalum"
  • 10. Erneux C., Miot F., Van Haastert P. J. M.Jastorff B., J. Cyclic Nucleotide Protein Phosphorylation Res., 10, 463 - 472 (1985), "The Binding of Cyclic Nucleotide Analogs to a Purified Cyclic GMP- Stimulated Phosphodiesterase from Bovine Adrenal Tissue"
  • 11. O'Brian C. A., Roczniak S. O., Bramson H. N., Baraniak J., Stec W. J.Kaiser E. T., Biochemistry, 21, 4371 - 4376 (1982), "A Kinetic Study of Interactions of (Rp)- and (Sp)-Adenosine Cyclic 3',5'-Phosphorothioates with Type II Bovine Cardiac Muscle Adenosine Cyclic 3',5'-Phosphate Dependent Protein Kinase"
  • 12. Rannels S. R.Corbin J. D., J. Biol. Chem., 255, 7085 - 7088 (1980), "Two Different Intrachain cAMP Binding Sites of cAMP-Dependent Protein Kinases"
  • 13. Corbin J. D., Sugden P. H., West L., Flockhart D. A., Lincoln T. M.McCarthy D., J. Biol. Chem., 253, 3997 - 4003 (1978), "Studies on the Properties and Mode of Action of the Purified ReguIatory Subunit of Bovine Heart Adenosine 3':5' Monophosphate-dependent Protein Kinase"