The protein kinase A activator Sp-cAMPS immobilized on agarose by an aminohexylamino spacer attached to position 8 of the ligand. Free Sp-cAMPS is only very slowly metabolized by corresponding phosphodiesterases and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase A compared to cAMP should result in potentially milder desorption conditions in affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This ligand is offered with a shorter spacer (Sp-8-AEA-cAMPS-Agarose, Cat. No. A 008) as well. Detailed technical information available.

Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.