0 Items - $0.00
Categories
A to Z

Rp-dGTP-α-S

2'- Deoxyguanosine- 5'- O- (1- thiotriphosphate), Rp isomer (Rp-dGTP-α-S); sodium salt
Cat. No.: D 030
    
CAS No.: [80902-29-0]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: D 030-05
Unit: 5 µmol / ~2.6 mg
$299.00

For other salt forms or a guaranteed higher purity please inquire. Useful for modulation of dGTP-responsive receptors and determination of their stereospecificity. Increased metabolic stability compared to dGTP. Not accepted by DNA polymerases. Detailed technical information available. References: Kunkel et al., Proc. Natl. Acad. Sci. USA, 78, 6734 - 6738 (1981); Abbotts et al., J. Biol. Chem., 263, 15094 - 15103 (1988).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6

Cat. No. D 030
CAS number [80902-29-0]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Molecular formula C₁₀H₁₆N₅O₁₂P₃S (free acid)
Molecular weight [g/mol] 523,3 (free acid)
Absorption max [nm] 252
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 14300
  • 1. Ryder S. P.Strobel S. A., Methods Enzymol., 18, 38 - 50 (1999), "Nucleotide Analog Interference Mapping"
  • 2. Arabshani A.Frey P. A., Biochem. Biophys. Res. Commun., 204, 150 - 155 (1994), "A Simplified Procedure for Synthesizing Nucleoside 1-Thiotriphosphates: dATP-alpha-S, dGTP-alpha-S, UTP-alpha-S and dTTP-alpha-S"
  • 3. Abbotts J., SenGupta D. N., Zon G.Wilson S. H., J. Biol. Chem., 263, 15094 - 15103 (1988), "Studies on the Mechanism of Escherichia coli DNA Polymerase I Large Fragment"
  • 4. Goody R. S.Isakov M., Tetrahedron Lett., 27, 3599 - 3602 (1986), "Simple Synthesis and Separation of the Diastereomers of alpha-Thio Analogs of Ribo- and Deoxyribo-di- and Triphosphates"
  • 5. Goody R., Isakov M.Labeit S., Ger. Offen. DE 3374, 546, 0 - 0 (1985), "DNA Sequencing Involving Alpha-thiodeoxyribonucleotide triphosphate-labeled DNA and Exonuclease Digestion, Synthesis of Alpha-thionucleotide triphosphates and Resolution of the Resulting Diastereomeric Mixture"
  • 6. Kunkel T. A., Eckstein F., Mildvan A. S., Koplitz R. M.Loeb L. A., Proc. Natl. Acad. Sci. USA, 78, 6734 - 6738 (1981), "Deoxynucleoside [1-thio]triphosphates Prevent Proofreading During in vitro DNA Synthesis"