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Sp-dGTP-α-S

2'- Deoxyguanosine- 5'- O- (1- thiotriphosphate), Sp isomer (Sp-dGTP-α-S); sodium salt
Cat. No.: D 031
    
CAS No.: [80902-28-9]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: D 031-05
Unit: 5 µmol / ~2.6 mg
$340.00

For other salt forms or a guaranteed higher purity please inquire. Useful for modulation of dGTP-responsive receptors and determination of their stereospecificity. Increased metabolic stability compared to dGTP. Accepted by DNA polymerase for incorporation of phosphorothioate into DNA. Detailed technical information available. References: Abbotts et al., J. Biol. Chem., 263, 15094 - 15103 (1988); Nakamaye et al., Nucl. Acids Res., 16, 9947 - 9959 (1988); Nyrén et al., Anal. Biochem., 244, 367 - 373 (1997).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6

Cat. No. D 031
CAS number [80902-28-9]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Molecular formula C₁₀H₁₆N₅O₁₂P₃S (free acid)
Molecular weight [g/mol] 523.3 (free acid)
Absorption max [nm] 252
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 14300
  • 1. Lutz S., Ostermeier M.Benkovic S. J., Nucl. Acids Res. e16, 29, 0 - 0 (2001), "Rapid Generation of Incremental Truncation Libraries for Protein Engineering Using Alpha-Phosphorothioate Nucleotides"
  • 2. Sauer S., Lechner D., Berlin K., Plancon C., Heuermann A., Lehrach H.Gut I. G., Nucleic Acids Res. 100e, 28, 0 - 0 (2000), "Full Flexibility Genotyping of Single Nucleotide Polymorphisms by the GOOD Assay"
  • 3. Sauer S., Lechner D., Berlin K., Lehrach H., Escary J.-L., Fox N.Gut I. G., Nucleic Acids Res. e13, 28, 0 - 0 (2000), "A Novel Procedure for Efficient Genotyping of Single Nucleotide Polymorphisms"
  • 4. Ryder S. P.Strobel S. A., Methods Enzymol., 18, 38 - 50 (1999), "Nucleotide Analog Interference Mapping"
  • 5. Xu S. H.Gaskin F., Biochim. Biophys. Acta, 1383, 111 - 122 (1998), "Probing the ATP Binding Site of Tubulin with Thiotriphosphate Analogues of ATP"
  • 6. Nyren P., Karamohamed S.Ronaghi M., Anal. Biochemistry, 244, 367 - 373 (1997), "Detection of Single-Base Changes Using a Bioluminometric Primer Extension Assay"
  • 7. Schachter J. B.Harden T. K., Br. J. Pharmacol., 121, 338 - 344 (1997), "An Examination of Deoxyadenosine 5' (alpha-thio)triphosphate as a Ligand to Define P2Y Receptors and its Selectivity as a low Potency Partial Agonist of the P2Y1 Receptor"
  • 8. Li H., Hardin C.Ramsay-Shaw B., J. Amer. Chem. Soc., 118, 6606 - 6614 (1996), "Hydrolysis of Thymidine Boranophosphate and Stepwise Deuterium Substitution of the Borane Hydrogens. 31P and 11B NMR Studies"
  • 9. Conrad F., Hanne A., Gaur R. K.Krupp G., Nucl. Acids Res., 23, 1845 - 1853 (1995), "Enzymatic Synthesis of 2'-modified Nucleic Acids: Identification of Important Phosphate and Ribose Moities in RNase P Substrates"
  • 10. Arabshani A.Frey P. A., Biochem. Biophys. Res. Commun., 204, 150 - 155 (1994), "A Simplified Procedure for Synthesizing Nucleoside 1-Thiotriphosphates: dATP-alpha-S, dGTP-alpha-S, UTP-alpha-S and dTTP-alpha-S"
  • 11. Olsen D. B., Sayers J. R.Eckstein F., Methods Enzymol., 217, 189 - 217 (1993), "Site-directed Mutagenesis of Single-stranded and Double-stranded DNA by Phosphorothioate Approach"
  • 12. Lee L. G., Connell C. R., Woo S. L., Cheng R. D., McArdle B. F., Fuller C. W., Halloran N. D.Wilson R. K., Nucleic Acids Res., 20, 2471 - 2483 (1992), "DNA Sequencing with Dye-labeled Terminators and T7 DNA Polymerase: Effect of Dyes and dNTPs on Incorporation of Dye-terminators and Probability Analysis of Termination Fragments"
  • 13. King P.Goodburn S., Nucl. Acids Res., 20, 1039 - 1044 (1992), "A Method for Sequence-specific Deletion Mutagenesis"
  • 14. Olsen D. B., Kotzorek G.Eckstein F., Biochemistry, 29, 9546 - 9551 (1990), "Investigation of the Inhibitory Role of Phosphorothioate Internucleotidic Linkages on the Catalytic Activity of the Restriction Endonuclease EcoRV"
  • 15. Abbotts J., SenGupta D. N., Zon G.Wilson S. H., J. Biol. Chem., 263, 15094 - 15103 (1988), "Studies on the Mechanism of Escherichia coli DNA Polymerase I Large Fragment"
  • 16. Nakamaye K. L., Gish G., Eckstein F.Vosberg H.-P., Nucl. Acids Res., 16, 9947 - 9959 (1988), "Direct Sequencing of Polymerase Chain Reaction Amplified DNA Fragments Through the Incorporation of Deoxynucleoside alpha-Thiotriphosphates"
  • 17. Gish G.Eckstein F., Science, 240, 1520 - 1522 (1988), "DNA and RNA Sequence Determination Based on Phosphorothioate Chemistry"
  • 18. Labeit S., Lehrach H.Goody R. S., Methods Enzymol., 155, 166 - 177 (1987), "DNA Sequencing Using Alpha-Thiodeoxynucleotides"
  • 19. Goody R. S.Isakov M., Tetrahedron Lett., 27, 3599 - 3602 (1986), "Simple Synthesis and Separation of the Diastereomers of alpha-Thio Analogs of Ribo- and Deoxyribo-di- and Triphosphates"
  • 20. Goody R., Isakov M.Labeit S., Ger. Offen. DE 3374, 546, 0 - 0 (1985), "DNA Sequencing Involving Alpha-thiodeoxyribonucleotide triphosphate-labeled DNA and Exonuclease Digestion, Synthesis of Alpha-thionucleotide triphosphates and Resolution of the Resulting Diastereomeric Mixture"
  • 21. Chen J.-T.Benkovic S. J., Nucl. Acids Res., 11, 3737 - 3751 (1983), "Synthesis and Separation of Diastereomers of Deoxynucleoside 5'- O- (1-thio)triphosphates"
  • 22. Vosberg H.-P.Eckstein F., J. Biol. Chem., 257, 6595 - 6599 (1982), "Effect of Deoxynucleoside Phosphorothioates Incorporated in DNA on Cleavage by Restriction Enzymes"
  • 23. Kunkel T. A., Eckstein F., Mildvan A. S., Koplitz R. M.Loeb L. A., Proc. Natl. Acad. Sci. USA, 78, 6734 - 6738 (1981), "Deoxynucleoside [1-thio]triphosphates Prevent Proofreading During in vitro DNA Synthesis"

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