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MANT-GppNHp

2'- / 3'- O- (N'- Methylanthraniloyl)guanosine- 5'- O- [(β, γ- imidotriphosphate] ( MANT-GppNHp ), sodium salt
Fluorescent form of the hydrolysis-resistant GppNHp
Cat. No.: M 047
    
CAS No.: [148821-01-6]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: M 047-01
Unit: 1 µmol / ~0.7 mg
$297.00

For other salt forms please inquire. Fluorescent analogue of the hydrolysis-resistant guanosine-5'-O-[(β, γ)- imido]triphosphate (λexc 350 nm; λem 442 nm), useful for research into GTP- dependent receptor proteins. The MANT fluorophore has a certain sensitivity for its environment and can change its spectral properties upon binding. Potent inhibitor of adenylate cyclase. Detailed technical information available. References: Herrmann et al., J. Biol. Chem., 270, 2901 - 2905 (1995); Gille. & Seifert, J. Biol. Chem., 278, 12672 - 12679 (2003); Zhang et al., J. Biol. Chem., 280, 33200 - 33205 (2005).

 

Vial containing 100 µl of 10 mM aqueous solution of pH 7.6

Cat. No. M 047
CAS number [148821-01-6]
Purity > 90% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₁₈H₂₄N₇O₁₄P₃ (free acid)
Molecular weight [g/mol] 655,4 (free acid)
Absorption max [nm] 252
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 21500
UV pH value 8
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  • 3. Zhang Z., Rehmann H., Price L. S., Riedl J.Bos J. L., J. Biol. Chem., 280, 33200 - 33205 (2005), "AF6 Negatively Regulates Rap1-induced Cell Adhesion"
  • 4. Gille A.Seifert R., J. Biol. Chem., 278, 12672 - 12679 (2003), "2'(3')-O-(N-Methylanthraniloyl)-substituted GTP Analogs: A Novel Class of Potent Competitive Adenylyl Cycalse Inhibitors"
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  • 6. Herrmann C., Martin G. A.Wittinghofer A., J. Biol. Chem., 270, 2901 - 2905 (1995), "Quantitative Analysis of the Complex between P21Ras and the Ras-Binding Domain of the Human Raf-1 Protein Kinase"