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ATP-γ-AmNS

Adenosine- 5'- O- triphosphoro- γ- 1- (5- sulfonic acid)naphthylamidate ( ATP-γ-AmNS ), sodium salt
Fluorest analogue of ATP
Cat. No.: A 176
    
CAS No.: [66492-86-2]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: A 176-05
Unit: 5 µmol / ~3.6 mg
$375.00

For other salt forms or a guaranteed higher purity please inquire. ATP-γ-AmNS is a fluorescent analogue of ATP with λem 460 nm (quantum yield = 0.63). Upon cleavage of the α-β-phosphoryl bond the emission maximum shifts to 475 nm. Detailed technical information available. References: Yarbrough, Biochem. Biophys. Res. Comm., 81, 35 - 41 (1978); Yarbrough & Bock, J. Biol. Chem., 255, 9907 - 9911 (1980).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6.

Cat. No. A 176
CAS number [66492-86-2]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₂₀H₂₃N₆O₁₅P₃S (free acid)
Molecular weight [g/mol] 712,4 (free acid)
Emission max [nm] 460
Absorption max [nm] 246
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 31000
  • 1. Wu F. Y.-H.Tyagi S. C., J. Biol. Chem., 262, 13147 - 13154 (1987), "Fluoresence Resonance Energy Transfer Studies on the Proximity Relationship between the Intrinsic Metal Ion and Substrate Binding Sites of Escherichia coli RNA polymerase"
  • 2. Pollack S. E.Auld D. S., Anal. Biochem., 127, 81 - 88 (1982), "Fluorescent Nucleotide Triphosphate Substrates for Snake Venom Phosphodiesterase"
  • 3. Yarbrough L. R.Bock J. L., J. Biol. Chem., 255, 9907 - 9911 (1980), "Stacking Interactions in Fluorescent Nucleotide Analogs Containing 1-Aminonaphthalene-5-Sulfonate at the Phosphoryl Terminus"
  • 4. Yarbrough L. R., Schlageck J. G.Baughman M., J. Biol. Chem., 254, 12069 - 12073 (1979), "Synthesis and Properties of Fluorescent Nucleotide Substrates for DNA-dependent RNA Polymerases"
  • 5. Schlageck J. G., Baughman M.Yarbrough L. R., J. Biol. Chem., 254, 12074 - 12077 (1979), "Spectroscopic techniques for Study of Phosphodiester Bond Formation by Escherichia coli RNA Polymerase"
  • 6. Yarbrough L. R., Biochem. Biophys. Res. Commun., 81, 35 - 41 (1978), "Synthesis and Properties of a New Fluorescent Analog of ATP: Adenosine-5'-Triphosphoro-Gamma-1-(5-Sulfonic Acid) Napthylamidate"