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APS

Adenosine- 5'- O- phosphosulfate ( APS ), sodium salt
Active form of sulfate
Cat. No.: A 061
    
CAS No.: [102029-95-8]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: A 061-10
Unit: 10 µmol / ~4.3 mg
$72.00

For the corresponding phosphorothioate-modified structures please inquire. APS is a sulfate ester of adenosine-5'-O-monophosphate (5'-AMP) and represents an active form of sulfate. Useful in pyrosequencing and bioluminescence applications. Detailed technical information and references available. References: Ronaghi et al., Science, 281, 363 - 365 (1998); Hofgen et al., Amino Acids, 20, 291 - 299 (2001); Eriksson et al., Anal. Biochem., 293, 67 - 70 (2001).

 

Vial containing 1 ml of 10 mM aqueous solution of pH 5.5.

Cat. No. A 061
CAS number [102029-95-8]
Purity guaranteed purity > 95% HPLC, usual purity > 98%
Salt form Sodium
Storage temperature -70°C / -94°F
Molecular formula C₁₀H₁₄N₅O₁₀PS (free acid)
Molecular weight [g/mol] 427.3 (free acid)
Absorption max [nm] 259
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 15000
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  • 2. Gandelman O. A., Church V. L., Moore C. A., Kiddle G., Carne C. A., Parmar S., Jalal H., Tisi L. C.Murray J. A. H., PloS One, 5, 1 - 13 (2010), "Novel Bioluminescent Quantitative Detection of Nucleic Acid Amplification in Real-Time"
  • 3. Hassibi A., Contag C., Vlad M. O., Hafezi M., Lee T. H., Davis R. W.Pourmand N., Biophys. Chem., 116, 175 - 185 (2005), "Bioluminescence Regenerative Cycle (BRC) System: Theoretical Considerations for Nucleic Acid Quantification Assays"
  • 4. Hofgen R., Kreft O., Willmitzer L.Hesse H., Amino Acids, 20, 291 - 299 (2001), "Manipulation of Thiol Contents in Plants"
  • 5. Eriksson J., Karamohamed S.Nyren P., Anal. Biochem., 293, 67 - 70 (2001), "Method for Real-Time Detection of Inorganic Pyrophosphatase Activity"
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  • 7. Zhang H.Leyh T. S., J. Am. Chem. Soc., 121, 8692 - 8697 (1999), "Alpha-Thio-APS: A Stereomechanistic Probe of Activated Sulfate Synthesis"
  • 8. Ronaghi M., van Bemmelen M. X. P.Nyren P., Science, 281, 363 - 365 (1998), "Real-Time Pyrophosphate Detection for DNA Sequencing"
  • 9. Yang M.Leyh T. S., Biochemistry, 36, 3270 - 3277 (1997), "Altering the Reaction Coordinate of the ATP Sulfurylase-GTPase Reaction"
  • 10. Nakamura J.Lou L., J. Biol. Chem., 270, 7347 - 7353 (1995), "Biochemical Characterization of Human GMP Synthetase"
  • 11. Ng K., D'Souza M., Callahan L., Geller D. H., Kearns A. E., Lyle S.Schwartz N. B., Anal. Biochem., 198, 60 - 67 (1991), "Synthesis and Utilization of a Nonhydrolyzable Phosphoadenosine Phosphosulfate Analog"