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TNP-ATP

2', 3'- O- (2, 4, 6- Trinitrophenyl)adenosine- 5'- O- triphosphate ( TNP-ATP ), sodium salt
Fluorescent analogue of ATP
Cat. No.: T 025
    
CAS No.: [120360-48-7]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: T 025-05
Unit: 5 µmol / ~3.6 mg
$207.00

For other salt forms please inquire. Fluorescent analogue of ATP with λexc 408 nm and λem 561 nm, which can substitute for ATP in the interaction with various enzymes and proteins. It is sensitive to indicators of local environment such as polarity and viscosity, and frequently exhibits a spectral shift and fluorescence enhancement upon binding to a protein. Potent, selective P2X receptor antagonist and potent inhibitor of soluble guanylyl cyclase (sGC). Detailed technical information available. References: Hiratsuka, T., Biochim. Biophys. Acta, 719, 509 - 517 (1982); Virginio et al., Mol. Pharmacol., 53, 969 - 973 (1998); Suryanarayana et al., J. Pharmacol. Exp. Ther., 330, 687 - 695 (2009).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 8

Cat. No. T 025
CAS number [120360-48-7]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 8
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₁₆H₁₇N₈O₁₉P₃ (free acid)
Molecular weight [g/mol] 718,3 (free acid)
Excitation max [nm] 408
Emission max [nm] 561
Absorption max [nm] 408
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 26400
UV pH value 8
  • 1. Suryanarayana S., G�ttle M., H�bner A., Gille A., Mou T.-C., Sprang S. R., Richter M.Seifert R., J. Pharmacol. Exp. Ther., 330, 687 - 695 (2009), "Differential Inhibition of Various Adenylyl Cyclase Isoforms and Soluble Guanylyl Cyclase by 2',3'-O-(2,4,6-Trinitrophenyl)-substituted Nucleoside 5'-triphosphates"
  • 2. Hiratsuka T., Eur. J. Biochem., 270, 3479 - 3485 (2003), "Fluorescent and Colored Trinitrophenylated Analogs of ATP and GTP"
  • 3. Dunn P. M., Liu M., Zhong Y., King B. F.Burnstock G., Br. J. Pharmacol., 130, 1378 - 1384 (2000), "Diinosine Pentaphosphate: an Antagonist which Discriminates between Recombinant P2X3 and P2X2/3 Receptors and between Two P2X Receptors in Rat Sensory Neurones"
  • 4. Virginio C., Robertson G., Surprenant A.North R. A., Mol. Pharmacol., 53, 969 - 973 (1998), "Trinitrophenyl-substituted Nucleotides are Potent Antagonists Selective for P2X1, P2X3, and heteromeric P2X2/3 Receptors"
  • 5. Chavan A. J., Haley B. E., Volkin D. B., Marfia K. E., Verticelli A. M., Bruner M. W., Draper J. P., Burke C. J.Middaugh C. R., Biochemistry, 33, 7193 - 7202 (1994), "Interaction of Nucleotides with Acidic Fibroblast Growth Factor (FGF- 1)"
  • 6. Hiratsuka T., Biochim. Biophys. Acta, 719, 509 - 517 (1982), "Biological Activities and Spectroscopic Properties of Chromophoric and Fluorescent Analogs of Adenine Nucleoside and Nucleotides, 2',3'-O-(2,4,6-Trinitrocyclohexadienylidene) Adenosine Derivatives"