0 Items - $0.00
Categories
A to Z

Sp-UTP-α-S

Uridine- 5'- O- (1-thiotriphosphate), Sp-isomer ( Sp-UTP-α-S ), sodium salt
Cat. No.: U 003
    
CAS No.: [71214-29-4]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: U 003-05
Unit: 5 µmol / ~2.5 mg
$238.00

For other salt forms or a guaranteed higher purity please inquire.
Useful for modulation of UTP-responsive receptors and determination of their stereospecificity. Increased metabolic stability compared to UTP. Accepted by RNA polymerases. Detailed technical information available. Reference: Armstrong et al., Biochemistry, 18, 4120 - 4123 (1979).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6

Cat. No. U 003
CAS number [71214-29-4]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Molecular formula C₉H₁₅N₂O₁₄P₃S (free acid)
Molecular weight [g/mol] 500,2 (free acid)
Absorption max [nm] 262
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 10000
  • 1. Jacobson K. A., Costanzi S., Ivanov A. A., Tchilibon S., Besada P., Gao Z.-G., Maddileti S.Harden T. K., Biochem. Pharmacol., 71, 540 - 549 (2006), "Structure Activity and Molecular Modeling Analyses of Ribose- and Base-modified Uridine 5'-triphosphate Analogues at the Human P2Y2 and P2Y4 Receptors"
  • 2. Koziolkiewicz M., Gendaszewska E., Maszewska M., Stein C. A.Stec W. J., Blood, 98, 995 - 1002 (2001), "The Mononucleotide-dependent, Nonantisense Mechanism of Action of Phosphodiester and Phosphorothioate Oligonucleotides Depends upon the Activity of an Ecto-5'-nucleotidase"
  • 3. Ryder S. P.Strobel S. A., Methods Enzymol., 18, 38 - 50 (1999), "Nucleotide Analog Interference Mapping"
  • 4. Schäfer R.Reiser G., Br. J. Pharmacol., 121, 913 - 922 (1997), "Characterization of 35S-ATP-alpha-S and 3H-alpha, beta-MEATP Binding Sites in Rat Brain Cortical Synaptosomes: Regulation of Ligand Binding by Divalent Cations"
  • 5. Greco N. J., Arterioscl. Thromb. Vasc. Biol., 17, 769 - 777 (1997), "Functional Expression of a P-2T ADP Receptor in Xenopus Oocytes Injected with Megakaryocyte (CMK 11-5) RNA"
  • 6. Prabhu N. S., Dinter-Gottlieb G.Gottlieb P. A., Nucl. Acids Res., 25, 5119 - 5124 (1997), "Single Substitutions of Phosphorothioates in the HDV Ribozyme G73 Define Regions Necessary for Optimal Self-cleaving Activity"
  • 7. Conrad F., Hanne A., Gaur R. K.Krupp G., Nucl. Acids Res., 23, 1845 - 1853 (1995), "Enzymatic Synthesis of 2'-modified Nucleic Acids: Identification of Important Phosphate and Ribose Moities in RNase P Substrates"
  • 8. Arabshani A.Frey P. A., Biochem. Biophys. Res. Commun., 204, 150 - 155 (1994), "A Simplified Procedure for Synthesizing Nucleoside 1-Thiotriphosphates: dATP-alpha-S, dGTP-alpha-S, UTP-alpha-S and dTTP-alpha-S"
  • 9. Buzayan J. M., van Tol H., Feldstein P. A.Bruening G., Nucl. Acids Res., 18, 4447 - 4451 (1990), "Identification of a Non-junction Phosphodiester That Influences an Autolytic Processing Reaction of RNA"
  • 10. Frey P. A., Tetrahedron, 38, 1541 - 1567 (1982), "Stereochemistry of Enzymatic Reactions of Phosphates"
  • 11. Eckstein F., Accounts of Chemical Research, 12, 204 - 210 (1979), "Phosphorothioate analogues of nucleotides"
  • 12. Armstrong R. A., Yee D.Eckstein F., Biochemistry, 18, 4120 - 4123 (1979), "Mechanistic Studies on Deoxyribonucleic Acid Dependent Ribonucleic Acid Polymerase from Escherichia coli Using Phosphorothioate Analogues. 2. The Elongation Reaction"
  • 13. Sheu K.-F. R., Richard J. P.Frey P. A., Biochemistry, 18, 5548 - 5556 (1979), "Stereochemical Courses of Nucleotidyltransferase and Phosphotransferase Action. Uridine Diphosphate Glucose Pyrophosphorylase, Galactose-1-phosphate Uridylyltransferase, Adenylate Kinase, and Nucleoside Diphosphate Kinase"
  • 14. Sheu K.-F. R.Frey P. A., J. Biol. Chem., 253, 3378 - 3380 (1978), "UDP-glucose Pyrophosphorylase"
  • 15. Eckstein F., Angew. Chem., 87, 179 - 212 (1975), "Untersuchung der Wirkungsweise von Enzymen mit Nucleosidphosphorothioaten"