The protein kinase G activator Sp-cGMPS
(Cat. No. G 017) immobilized on agarose by an aminoethylthio spacer attached to position 8 of the ligand. Free Sp-cGMPS is only very slowly metabolized and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase G compared to cGMP should result in potentially milder desorption conditions in affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. Detailed technical information available.Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.