Alternative structural projections:
The protein kinase G activator Sp-cGMPS (Cat. No. G 017) immobilized on agarose by an aminoethylthio spacer attached to position 8 of the ligand. Free Sp-cGMPS is only very slowly metabolized and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase G compared to cGMP should result in potentially milder desorption conditions in affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. Detailed technical information available.
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Appearance: Suspension in 30 mM Na2HPO4 buffer (pH 7).
| Cat. No. | A 010 |
| CAS number | [pending] |
| Appearance | Suspension in 30 mM Na₂HPO₄ buffer (pH 7) |
| Storage temperature | 4°C / 39°F |
| Modifications | 8-cGMP |
- 1. Wolfe L., Corbin J. D.Francis S. H., J. Biol. Chem., 264, 7734 - 7741 (1989), "Characterization of a Novel Isozyme of cGMP-dependent Protein Kinase From Bovine Aorta"
