The protein kinase A activator Sp-cAMPS
(Cat. No. A 003) immobilized on agarose by an aminoethylamino spacer attached to position 8 of the ligand. Free Sp-cAMPS is only very slowly metabolized by mammalian phosphodiesterases and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase A compared to cAMP results in milder desorption conditions during affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This ligand is offered with a longer spacer (Sp-8-AHA-cAMPS-Agarose
, Cat. No. A 013) as well. Detailed technical information available. Reference: Bertinetti et al., BMC Chem. Biol
(2009). Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.