Alternative structural projections:
The protein kinase A activator Sp-cAMPS (Cat. No. A 003) immobilized on agarose by an aminoethylamino spacer attached to position 8 of the ligand. Free Sp-cAMPS is only very slowly metabolized by mammalian phosphodiesterases and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase A compared to cAMP results in milder desorption conditions during affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This ligand is offered with a longer spacer (Sp-8-AHA-cAMPS-Agarose, Cat. No. A 013) as well. Detailed technical information available. Reference: Bertinetti et al., BMC Chem. Biol, 9 (2009).
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
| Cat. No. | A 008 |
| CAS number | [pending] |
| Storage temperature | 4°C / 39°F |
| cAMP modified at the exocyclic oxygen | axial (Sp-) |
| Modifications | 8-cAMP |
- 1. Chepurny O. G., Bertinetti D., Diskar M., Leech C. A., Afshari P., Tsalkova T., Cheng X., Schwede F., Genieser H.-G., Herberg F. W.Holz G. G., Mol. Endocrinol., 27, 1267 - 1282 (2013), "Stimulation of Proglucagon Gene Expression by Human GPR119 in Enteroendocrine L-cell Line GLUTag"
- 2. Hanke S. E., Bertinetti D., Badel A., Schweinsberg S., Genieser H.-G.Herberg F. W., N. Biotechnol., 28, 294 - 301 (2011), "Cyclic Nucleotides as Affinity Tools: Phosphorothioate cAMP Analogues Adress Specific PKA Subproteomes"
- 3. Bertinetti D., Schweinsberg S., Hanke S. E., Schwede F., Bertinetti O., Drewianka S., Genieser H.-G.Herberg F. W., BMC Chem Biol, 9, 0 - 0 (2009), "Chemical tools selectively target components of the PKA system"
- 4. Moll D., Prinz A., Brendel C. M., Berrera M., Guske K., Zaccolo M., Genieser H.-G.Herberg F. W., BMC Biochem., 9, 18 - 18 (2008), "Biochemical Characterization and Cellular Imaging of a Novel, Membrane Permeable Fluorescent cAMP Analog"
- 5. Iwitzki F., van Bemmelen M. X. P., Genieser H.-G.Jastorff B., Proc. Intl. Conf. Second Messengers Phosphoprot. UK, 0, 0 - 0 (1992), "New Affinity Materials for Cyclic AMP Binding Proteins"
- 6. Iwitzki F., van Bemmelen M. X. P., Genieser H.-G.Jastorff B., 3rd Swed.- Germ. Workshop Nucleic Acid Synth. Struct. Funct. Sweden, 0, 0 - 0 (1992), "cAMP Analogues Designed for Affinity Chromatography"
- 7. Dills W. L., Beavo J. A., Bechtel P. J.Krebs E. G., Biochem. Biophys. Res. Commun., 62, 70 - 77 (1975), "Purification of Rabbit Skeletal Muscle Protein Kinase Regulatory Subunit Using Cyclic Adenosine- 3': 5'- Monophosphate Affinity Chromatography"
