The protein kinase A activator Sp-cAMPS (Cat. No., A 003) immobilized on agarose by an aminoethyl spacer attached to position 6 of the ligand. Free Sp-cAMPS is only very slowly metabolized by corresponding phosphodiesterases and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase A compared to cAMP results in milder desorption conditions during affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This ligand is offered with a longer spacer (Sp-6-AH-cAMPS-Agarose, Cat. No. A 108, p. 72) as well. Detailed technical information available.
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.