The metabolically stable protein kinase A inhibitor Rp-cAMPS (Cat. No. A 002) immobilized on agarose by an aminoethylamino spacer attached to position 8 of the ligand. Free Rp-cAMPS binds to the protein kinase A holoenzyme, but prevents dissociation to free catalytic and regulatory subunits. In addition, it is not hydrolized by mammalian phosphodiesterases. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinase A (presumably as its holoenzyme), phosphodiesterases and others. This ligand is offered with a longer spacer (Rp-8-AHA-cAMPS-Agarose, Cat. No. A 012) as well. Detailed technical information available. Reference: Peter et al., Eur. J. Pain, 21, 1072 - 1086 (2017).
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Appearance: Suspension in 30 mM Na2HPO4 buffer (pH 7).
|Cat. No.||A 007|
|Appearance||Suspension in 30 mM Na₂HPO₄ buffer (pH 7)|
|Storage temperature||4°C / 39°F|
|cAMP modified at the exocyclic oxygen||equatorial (Rp-)|