The metabolically stable protein kinase A inhibitor Rp-cAMPS
(Cat. No. A 002) immobilized on agarose by an aminoethylamino spacer attached to position 8 of the ligand. Free Rp-cAMPS binds to the protein kinase A holoenzyme, but prevents dissociation to free catalytic and regulatory subunits. In addition, it is not hydrolized by mammalian phosphodiesterases. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinase A (presumably as its holoenzyme), phosphodiesterases and others. This ligand is offered with a longer spacer (Rp-8-AHA-cAMPS-Agarose
, Cat. No. A 012) as well. Detailed technical information available.Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.