Fluorescent analogue of adenosine, λexc 300 nm, λem 410 nm at pH 7. Detailed technical information available. References: Scheller & Sigel, J. Am. Chem. Soc., 105, 3005 – 3014 (1983); Miura et al., Anal. Biochem., 196, 84 – 88 (1991); Srivastava et al., Nucl. Acids Res., 22, 1296 - 1304 (1994).
| Cat. No. | E 011 |
| CAS number | [39007-51-7] |
| Purity | > 95% HPLC |
| Storage temperature | -20°C / -4°F |
| Light-sensitive | True |
| Molecular formula | C₁₂H₁₃N₅O₄ |
| Molecular weight [g/mol] | 291.3 |
| Excitation max [nm] | 300 |
| Emission max [nm] | 410 |
| Absorption max [nm] | 275 |
| Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] | 6000 |
- 1. Song E.-K., Park H.-J., Kim J.-S., Lee H.-H., Kim U.-H.Han M.-K., J. Biochem. Biophys. Methods, 63, 161 - 169 (2005), "A Novel Fluorometric Assay for ADP-ribose Pyrophosphatase Activity"
- 2. Bobalova J., Bobal P.Mutafova-Yambolieva V. N., Anal. Biochem., 305, 269 - 276 (2002), "High-Performance Liquid Chromatographic Technique for Detection of a Fluorescent Analogue of ADP-Ribose in Isolated Blood Vessel Preparation"
- 3. Schweitzer K., Mayr G. W.Guse A. H., Anal. Biochem., 299, 218 - 226 (2001), "Assay for ADP-Ribosyl Cyclase by Reverse-Phase High-Performance Liquid Chromatography"
- 4. Birnbaum K. B., Stolarski R.Shugar D., BBA, 1200, 55 - 63 (1994), "Structure and Conformation of the Cyclic Phosphate of Ganciclovir, a Broad-spectrum Antiviral Agent"
- 5. Srivastava S. C., Raza S. K.Misra R., Nucl. Acids Res., 22, 1296 - 1304 (1994), "1,N6-Etheno Deoxy and Ribo Adenosine and 3,N4-Etheno Deoxy and Ribo Cytidine Phosphoramidites. Strongly Fluorescent Structures for Selective Introduction in Defined Sequence DNA and RNA Molecules"
- 6. Miura K., Okumura M., Yukimura T.Yamamoto K., Anal. Biochem., 196, 84 - 88 (1991), "Fluorometric Determination of Plasma Adenosine Concentrations Using High-Performance Liquid Chromatography"
- 7. Scheller K. H.Siegel H., J. Am. Chem. Soc., 105, 3005 - 3014 (1983), "Molecular Properties of 1, N6-Ethenoadenosine in Comparison with Adenosine: Self-Association, Protonation, Metal Ion Complexation, and Tryptophan-Adduct Formation. A Study on e-Adenosine Using Proton Nuclear Magnetic Resonance, Ultraviolet Spectrophotometry, and Potentiometric pH Titration"
- 8. Dutta S. P., Mittelman A.Chheda G. B., Biochem. Med., 23, 179 - 184 (1980), "Metabolism of 1,N6-Ethenoadenosine"
