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3'-O-Ac-dCTP

3'- O- Acetyl- 2'- deoxycytidine- 5'- O- triphosphate ( 3'-O-Ac-dCTP )
Ribose-modified dCTP analogue
Cat. No.: A 277
    
CAS No.: [90290-80-5]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: A 277-05
Unit: 5 µmol / ~2.5 mg
$165.00

For other salt forms or a guaranteed higher purity please inquire. 3'-O-Ac-dCTP is an analogue of dCTP with blocked 3’-hydroxy function, which can be used as a transient, pH-sensitive chain terminator during DNA polymerization. The acetyl group of 3'-O-Ac-dCTP is sensitive towards alkaline conditions and can be split off at pH >12.5 within 30 min. to allow further subsequent polymerization steps. References: Marián, M., Microchem. J., 29, 219 - 227 (1984).

 Vial containing 500 µl of 10 mM aqueous solution of pH 7.6.

Cat. No. A 277
CAS number [90290-80-5]
Purity >95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution
Storage temperature -70°C / -94°F
Molecular formula C₁₁H₁₈N₃O₁₄P₃ (free acid)
Molecular weight [g/mol] 509.2 (free acid)
Absorption max [nm] 271
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 9000
UV pH value 7
Solvent for UV data Water