For other salt forms or a guaranteed higher purity please inquire. 3'-O-Ac-dATP is an analogue of dATP with blocked 3’-hydroxy function, which can be used as a transient, pH-sensitive chain terminator during DNA polymerization. The acetyl group of 3'-O-Ac-dATP is sensitive towards alkaline conditions and can be split off at pH >12.5 within 30 min. to allow further subsequent polymerization steps. References: Hansbury et al., Biochim. Biophys. Acta, 199, 322 – 329 (1970); Babkina et al., Izv. Sib. Otd. Akad. Nauk SSSR, Ser. Khim. Nauk, 128 - 132 (1975); Marián, M., Microchem. J., 29, 219 - 227 (1984); Metzker et al., Nucl. Acid Res., 22, 4259 – 4267 (1994).
Vial containing 500 µl of 10 mM aqueous solution of pH 7.6.
Cat. No. | A 276 |
CAS number | [28120-63-0] |
Purity | >95% HPLC |
Salt form | Sodium |
Appearance | 10 mM aqueous solution |
Storage temperature | -70°C / -94°F |
Molecular formula | C₁₂H₁₈N₅O₁₃P₃ (free acid) |
Molecular weight [g/mol] | 533.2 (free acid) |
Absorption max [nm] | 15000 |
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] | 259 |
UV pH value | 7 |
Solvent for UV data | Water |