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3'-O-Ac-dATP

3'- O- Acetyl- 2'- deoxyadenosine- 5'- O- triphosphate ( 3'-O-Ac-dATP )
Ribose-modified dATP analogue
Cat. No.: A 276
    
CAS No.: [28120-63-0]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: A 276-05
Unit: 5 µmol / ~2.7 mg
$145.00

For other salt forms or a guaranteed higher purity please inquire. 3'-O-Ac-dATP is an analogue of dATP with blocked 3’-hydroxy function, which can be used as a transient, pH-sensitive chain terminator during DNA polymerization. The acetyl group of 3'-O-Ac-dATP is sensitive towards alkaline conditions and can be split off at pH >12.5 within 30 min. to allow further subsequent polymerization steps. References: Hansbury et al., Biochim. Biophys. Acta, 199, 322 – 329 (1970); Babkina et al., Izv. Sib. Otd. Akad. Nauk SSSR, Ser. Khim. Nauk, 128 - 132 (1975); Marián, M., Microchem. J., 29, 219 - 227 (1984); Metzker et al., Nucl. Acid Res., 22, 4259 – 4267 (1994).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6.

Cat. No. A 276
CAS number [28120-63-0]
Purity >95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution
Storage temperature -70°C / -94°F
Molecular formula C₁₂H₁₈N₅O₁₃P₃ (free acid)
Molecular weight [g/mol] 533.2 (free acid)
Absorption max [nm] 15000
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 259
UV pH value 7
Solvent for UV data Water