Precursor for the preparation of the corresponding radio-labelled triphosphate to be used in chemical genetics approach (compare: Habelhah et al., J. Biol. Chem., 276, 18090 - 18095 (2001)). The corresponding cold triphosphate is offered as well (Cat. No. B 024). Detailed technical information available. References: Shah et al., Proc. Natl. Acad. Sci. USA, 94, 3565 - 3570 (1997); Provance et al., Proc. Natl. Acad. Sci. USA, 101, 1868 – 1873 (2004).
Vial containing 500 µl of 10 mM aqueous solution of pH 7.6
| Cat. No. | B 023 |
| CAS number | [40811-89-0] |
| Purity | > 95% HPLC |
| Salt form | Sodium |
| Appearance | 10 mM aqueous solution of pH 7.6 |
| Storage temperature | -70°C / -94°F |
| Molecular formula | C₁₇H₂₁N₅O₁₀P₂ (free acid) |
| Molecular weight [g/mol] | 517.3 (free acid) |
| Absorption max [nm] | 269 |
| Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] | 20500 |
- 1. Provance D. W., Gourley C. R., Silan C. M., Cameron L. C., Shokat K. M., Goldenring J. R., Shah K., Gillespie P. G.Mercer J. A., Proc. Natl. Acad. Sci., 101, 1868 - 1873 (2004), "Chemical-genetic Inhibition of a Sensitized Mutant Myosin Vb Demonstrates a Role in Peripheral-pericentriolar Membrane Traffic"
- 2. Liu Y., Kung C., Fishburn J., Ansari A. Z., Shokat K. M.Hahn S., Mol. Cell. Biol., 24, 1721 - 1735 (2004), "Two Cyclin-Dependent Kinases Promote RNA Polymerase II Trancription and Formation of the Scaffold Complex"
- 3. Gillespie P. G., Gillespie S. K. H., Mercer J. A., Shah K.Shokat K. M., J. Biol. Chem., 274, 31373 - 31381 (1999), "Engineering of the Myosin-Iß Nucleotide-binding Pocket to Create Selective Sensitivity to N6-modified ADP Analogs"
- 4. Shah K., Liu Y., Deirmengian C.Shokat K. M., Proc. Natl. Acad. Sci. USA, 94, 3565 - 3570 (1997), "Engineering Unnatural Nucleotide Specificity for Rous Sarcoma Virus Tyrosine Kinase to Uniquely Label its Direct Substrates"
