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8-N₃-ATP

8- Azidoadenosine- 5'- O- triphosphate ( 8-N₃-ATP ), sodium salt
ATP analogue with photoaffinity label
Cat. No.: A 043
    
CAS No.: [53696-59-6]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: A 043-05
Unit: 5 µmol / ~2.7 mg
$128.00

For other salt forms please inquire. Useful for photoaffinity labelling of ATP binding proteins and for incorporation into RNA for labelling purposes. Detailed technical information available. References: Haley & Hoffmann, Proc. Nat. Acad. Sci. USA, 71, 3367 - 3371 (1974); Potter et al., Methods Enzymol., 91, 613 - 633 (1983).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6

Cat. No. A 043
CAS number [53696-59-6]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₁₀H₁₅N₈O₁₃P₃ (free acid)
Molecular weight [g/mol] 548.2 (free acid)
Absorption max [nm] 281
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 13000
UV pH value 6
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  • 2. Daniel J. M., McCombie G., Wendt S.Zenobi R., Am. Soc. Mass Spect., 14, 442 - 448 (2003), "Mass Spectrometric Determination of Association Constants of Adenylate Kinase with Two Nonconvalent Inhibitors"
  • 3. Jang D. O., Kim J., Cho D. H.Chung C.-M., Tetrahedron, 42, 1073 - 1075 (2001), "Radical Deoxygenation of Alcohols via Their Trifluoroacetate Derivatives with Diphenylsilane"
  • 4. Ortoleva-Donnelly L., Kronman M.Strobel S. A., Biochemistry, 37, 12933 - 12942 (1998), "Identifying RNA Minor Groove Tertiary Contacts by Nucleotide Analogue Interference Mapping with N#-Methylguanosine"
  • 5. Maruta S., Ohki T., Kambara T.Ikebe M., Eur. J. Biochem., 256, 229 - 237 (1998), "Characterization of the Interaction of Myosin with ATP Analogues Having the Syn Conformation with Respect to the Adenine-Ribose Bond"
  • 6. Chavan A. J., Haley B. E., Volkin D. B., Marfia K. E., Verticelli A. M., Bruner M. W., Draper J. P., Burke C. J.Middaugh C. R., Biochemistry, 33, 7193 - 7202 (1994), "Interaction of Nucleotides with Acidic Fibroblast Growth Factor (FGF- 1)"
  • 7. Rajagopalan K., Chavan A. J., Haley B. E.Watt D. S., J. Biol. Chem., 268, 14230 - 14238 (1993), "Synthesis and Application of Bidentate Photoaffinity Cross-linking Reagents"
  • 8. Haley B. E., Methods in Enzymology, 200, 477 - 487 (1991), "Nucleotide Photoaffinity Labeling of Protein Kinase Subunits"
  • 9. Kwiatkowski A. P.King M. M., Biochemistry, 26, 7636 - 7640 (1987), "Mapping of the Adenosine 5'-Triphosphate Binding Site of Type II Calmodulin-Dependent Protein Kinase"
  • 10. Potter R. L.Haley B. E., Methods Enzymol., 91, 613 - 633 (1983), "Photoaffinity Labeling of Nucleotide Binding Sites with 8-Azidopurine Analogs: Techniques and Applications"
  • 11. Czarnecki J., Geahlen R.Haley B. E., Methods Enzymol., 56, 642 - 653 (1979), "Synthesis and Use of Azido Photoaffinity Analogs of Adenine and Guanine Nucleotides"
  • 12. Haley B. E.Hoffman J. F., Proc. Nat. Acad. Sci. USA, 71, 3367 - 3371 (1974), "Interactions of a Photo-Affinity ATP Analog with Cation-Stimulated Adenosine Triphosphatases of Human Red Cell Membranes"