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MANT-GDP

2'- / 3'- O- (N'- Methylanthraniloyl)guanosine- 5'- O- diphosphate ( MANT-GDP ), sodium salt
Fluorescent analogue of 5'-GDP
Cat. No.: M 041
    
CAS No.: [148821-02-7]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: M 041-05
Unit: 5 µmol / ~2.9 mg
$185.00

For other salt forms please inquire. Fluorescent analogue of guanosine-5’-diphosphate (λexc 350 nm; λem 442 nm), useful for research into GDP-dependent receptor proteins. The MANT fluorophore has a certain sensitivity for its environment and can change its spectral properties upon binding (compare: Hiratsuka, Biochim. Biophys. Acta, 742, 496 - 508 (1983)). Detailed technical information available. References:  Wieden et al., J. Biol. Chem., 277, 6032 - 6036 (2002); Rehmann, Methods Enzymol., 407, 159 - 173 (2006).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6

Cat. No. M 041
CAS number [148821-02-7]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₁₈H₂₂N₆O₁₂P₂ (free acid)
Molecular weight [g/mol] 576.4 (free acid)
Excitation max [nm] 350
Emission max [nm] 442
Absorption max [nm] 252
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 22600
UV pH value 8
  • 1. Chepurny O. G., Bertinetti D., Diskar M., Leech C. A., Afshari P., Tsalkova T., Cheng X., Schwede F., Genieser H.-G., Herberg F. W.Holz G. G., Mol. Endocrinol., 27, 1267 - 1282 (2013), "Stimulation of Proglucagon Gene Expression by Human GPR119 in Enteroendocrine L-cell Line GLUTag"
  • 2. Seifert R.Dove S., Pharmacol. Therapeut., 140, 200 - 212 (2013), "Inhibitors of Bacillus anthracis Edema Factor"
  • 3. Rehmann H., Sci. Rep., 3: 3032, 0, 0 - 0 (2013), "Epac-Inhibitors: Facts and Artefacts"
  • 4. Geduhn J., Dove S., Shen Y., Tang W.-J., König B.Seifert R., J. Pharmacol. Exp. Ther., 336, 104 - 115 (2011), "Bis-Halogen-Anthraniloyl-Substituted Nucleoside 5´-Triphosphate as Potent and Selective Inhibitors of Bordetella Pertussis Adenylyl Cyclase Toxin"
  • 5. Tsalkova T., Gribenko A. V.Cheng X., Assay Drug Dev. Technol., 9, 88 - 91 (2010), "Exchange Protein Directly Activated by Cyclic AMP Isoform 2 is not a Direct Target of Sulfonylurea Drugs"
  • 6. Tsalkova T., Blumenthal D. K., Mei F. C., White M. A.Cheng X. D., J. Biol. Chem., 284, 23644 - 23651 (2009), "Mechanism of Epac Activation Structural and Functional Analyses of Epac2 Hinge Mutants with Constitutive and Reduced Activities"
  • 7. Branham M. T., Bustos M. A., De Blas G. A., Rehmann H., Zarelli V. E., Trevino C. L., Darszon A., Mayorga L. S.Tomes C. N., J. Biol. Chem., 284, 24825 - 24839 (2009), "Epac Activates the Small G Proteins Rap1 and Rab3A to Achieve Exocytosis"
  • 8. Holz G. G., Chepurny O. G.Schwede F., Cell. Signal., 20, 10 - 20 (2008), "Epac-selective cAMP Analogs: New Tools with which to Evaluate the Signal Transduction Properties of cAMP-regulated Guanine Nucleotide Exchange Factors"
  • 9. Wang J. L., Guo J.-X., Zhang Q.-Y., Wu J. J.-Q., Seifert R.Lushington G. H., Bioorg. Med. Chem., 15, 2993 - 3002 (2007), "A Conformational Transition in the Adenylyl Cyclase Catalytic Site Yields Different Binding Modes for Ribosyl-modified and Unmodified Nucleotide Inhibitors"
  • 10. Kuiperij H. B., Rehmann H.Zwartkruis F. J. T., Regulators and Effectors of Small GTPases: RAS Family, 407, 174 - 186 (2006), "Biochemistry of the Rap-specific Guanine Nucleotide Exchange Factors PDZ-GEF1 and -2"
  • 11. Rehmann H., Methods Enzymol., 407, 159 - 173 (2006), "Characterization of the Activation of the Rap-specific Exchange Factor Epac by Cyclic Nucleotides"
  • 12. Rehmann H., Wittinghofer A.Bos J. L., Manuskript (Status 09.08.05), 0, 0 - 0 (2005), "Structure of the cAMP Responsive Exchange Factor Epac2 in its Auto-inhibited State"
  • 13. Rehmann H., Methods Enzymol., 407, 159 - 173 (2005), "Characterization of the Activation of the Rap-specific Exchange Factor Epac by Cyclic Nucleotides"
  • 14. Kuiperij H. B., de Rooij J., Rehmann H., van Triest M., Wittinghofer A., Bos J. L.Zwartkruis F. J. T., Biochim. Biophys. Acta-Mol. Cell Res., 1593, 141 - 149 (2003), "Characterisation of PDZ-GEFs, a Family of Guanine Nucleotide Exchange Factors Specific for Rap1 and Rap2"
  • 15. Mohr D., Wintermeyer W.Rodnina M. V., Biochemistry, 41, 12520 - 12528 (2002), "GTPase Activation of Elongation Factors Tu and G on the Ribosome"
  • 16. Wieden H.-J., Gromadski K., Rodnin D.Rodnina M. V., J. Biol. Chem., 277, 6032 - 6036 (2002), "Mechanism of Elongation Factor (EF)-Ts-catalyzed Nucleotide Exchange Factor in EF-Tu. Contribution of Contacts at the Guanine Base"
  • 17. Thomä N. H., Iakovenko A., Kalinin A., Waldmann H., Goody R. S.Alexandrov K., Biochemistry, 40, 268 - 274 (2001), "Allosteric Regulation of Substrate Binding and Product Release in Geranylgeranyltransferase Type II"
  • 18. Lin B., Skidmore J. M., Bhatt A., Pfeffer S. M., Pawloski L.Maddock J. R., Mol. Microbiol., 39, 924 - 934 (2001), "Alanine Scan Mutagenesis of the Switch I Domain of the Caulobacter Crescentus CgtA Protein Reveals Critical Amino Acids Required for the in Vivo Function"
  • 19. Jagath J. R., Rodnina M. V., Lentzen G.Wintermeyer W., Biochemistry, 37, 15408 - 15413 (1998), "Interaction of Guanine Nucleotides with the Signal Recognition Particle from Escherichia coli"
  • 20. Yang M.Leyh T. S., Biochemistry, 36, 3270 - 3277 (1997), "Altering the Reaction Coordinate of the ATP Sulfurylase-GTPase Reaction"
  • 21. Jezewska M. J., Kim U. S.Bujalowski W., Biophysical J., 71, 2075 - 2086 (1996), "Interactions of Escherichia Coli Primary Replicative Helicase DNAB Protein with Nucleotide Cofactors"
  • 22. Simon I., Zerial M.Goody R. S., J. Biol. Chem., 271, 20470 - 20478 (1996), "Kinetics of Interaction of Rab5 and Rab7 with Nucleotides and Magnesium Ions"
  • 23. Brownbridge G. G., Lowe P. N., Moore K. J. M., Skinner R. H.Webb M. R., J. Biol. Chem., 268, 10914 - 10919 (1993), "Interaction of GTPase Activating Proteins (GAPs) with p21(ras) Measured by a Novel Fluorescence Anisotropy Method"
  • 24. Hazlett T. L., Moore K. J. M., Lowe P. N., Jameson D. M.Eccleston J. F., Biochemistry, 32, 13575 - 13583 (1993), "Solution Dynamics of p21ras Proteins Bound with Fluorescent Nucleotides: a Time-resolved Fluorescence Study"
  • 25. Neal S. E., Eckstein F.Webb M. R., Proc. Natl. Acad. Sci., 87, 3562 - 3565 (1990), "Hydrolysis of GTP by p21NRAS, the NRAS Protooncogene Product, is Accompanied by a Conformational Change in the Wild-Type Protein: Use of a Single Fluorescent Probe"
  • 26. John J., Sohmen R., Feuerstein J., Linke R., Wittinghofer A.Goody R. S., Biochemistry, 29, 6058 - 6065 (1990), "Kinetics of Interaction of Nucleotides with Nucleotide-Free H-ras p21"
  • 27. Hiratsuka T., Biochim. Biophys. Acta, 742, 496 - 508 (1983), "New Ribose-modified Fluorescent Analogs of Adenine and Guanine Nucleotides Available as Substrates for Various Enzymes"

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