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MANT-ATP

2'-/3'- O- (N- Methylanthraniloyl)adenosine- 5'- O- triphosphate ( MANT-ATP ), sodium salt
Fluorescent analogue of ATP
Cat. No.: M 030
    
CAS No.: [151481-86-6]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: M 030-05
Unit: 5 µmol / ~3.2 mg
$247.00

For other salt forms please inquire. Fluorescent analogue of adenosine-5’-triphosphate (λexc 350 nm; λem 446 nm), useful for research into ATP-dependent receptor proteins. The MANT fluorophore has a certain sensitivity for its environment and can change its spectral properties upon binding. Detailed technical information available. References: Hiratsuka, Biochim. Biophys. Acta, 742, 496 - 503 (1983); Woodward et al., Biochemistry, 30, 422 - 430 (1991); Mocz et al., Biochemistry, 37, 9862 - 9869 (1998). Potent inhibitor of adenylate cyclase (Mou et al., J. Biol. Chem., 280, 7253 - 7261 (2001).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6

Cat. No. M 030
CAS number [151481-86-6]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₁₈H₂₃N₆O₁₄P₃ (free acid)
Molecular weight [g/mol] 640.3 (free acid)
Excitation max [nm] 350
Emission max [nm] 446
Absorption max [nm] 255
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 23300
UV pH value 8
  • 1. Seifert R.Dove S., Pharmacol. Therapeut., 140, 200 - 212 (2013), "Inhibitors of Bacillus anthracis Edema Factor"
  • 2. Spangler C., Kaever V.Seifert R., J. Pharmacol. Exp. Ther., 336, 234 - 241 (2011), "Interaction of the Diguanylate Cyclase YdeH of Escheria Coli with 2´,(3´)-Substituted Purine and Pyrimidine Nucleotides"
  • 3. Geduhn J., Dove S., Shen Y., Tang W.-J., König B.Seifert R., J. Pharmacol. Exp. Ther., 336, 104 - 115 (2011), "Bis-Halogen-Anthraniloyl-Substituted Nucleoside 5´-Triphosphate as Potent and Selective Inhibitors of Bordetella Pertussis Adenylyl Cyclase Toxin"
  • 4. Göttle M., Dove S., Kees F., Schlossmann J., Geduhn J., König B., Shen Y., Tang W.-J., Kaever V.Seifert R., Biochemistry, 49, 5494 - 5503 (2010), "Cytidylyl and Uridylyl Cyclase Activity of Bacillus anthracis Edema Factor and Bordetella pertussis CyaA"
  • 5. Jiang M.Chen G., J. Neurosci., 29, 8063 - 8074 (2009), "Ca2+ Regulation of Dynamin-Independent Endocytosis in Cortical Astrocytes"
  • 6. Striedinger K., Meda P.Scemes E., Glia, 55, 652 - 662 (2007), "Exocytosis of ATP from Astrocyte Progenitors Modulates Spontaneous Ca2+ Oscillations and Cell Migration"
  • 7. Zhang Z., Chen G., Zhou W., Song A., Xu T., Luo Q., Wang W., Gu X.-S.Duan S., Nat. Cell Biol., 9, 945 - 953 (2007), "Regulated ATP Release from Astrocytes through Lysosome Exocytosis"
  • 8. Wang J. L., Guo J.-X., Zhang Q.-Y., Wu J. J.-Q., Seifert R.Lushington G. H., Bioorg. Med. Chem., 15, 2993 - 3002 (2007), "A Conformational Transition in the Adenylyl Cyclase Catalytic Site Yields Different Binding Modes for Ribosyl-modified and Unmodified Nucleotide Inhibitors"
  • 9. Cunliffe J. M., Sunahara R. K.Kennedy R. T., Anal. Chem., 78, 1731 - 1738 (2006), "Detection of Adenylyl Cyclase Activity Using a Fluorescent ATP Substrate and Capillary Electrophoresis"
  • 10. Mou T.-C., Gille A., Fancy D. A., Seifert R.Sprang S. R., J. Biol. Chem., 280, 7253 - 7261 (2005), "Structural Basis for the Inhibition of Mammalian Membrane Adenylyl Cyclase by 2'(3')-O-(N-Methylanthraniloyl)-guanosine 5'-triphosphate"
  • 11. Ni D. Q., Shaffer J.Adams J. A., Protein Sci., 9, 1818 - 1827 (2000), "Insights into Nucleotide Binding in Protein Kinase A Using Fluorescent Adenosine Derivatives"
  • 12. Oiwa K., Eccleston J. F., Anson M., Kikumoto M., Davis C. T., Reid G. P., Ferenczi M. A., Corrie J. E. T., Yamada A., Nakayama H.Trentham D. R., Biophys. J., 78, 3048 - 3071 (2000), "Comparative Single-Molecule and Ensemble Myosin Enzymology: Sulfoindocyanine ATP and ADP Derivatives"
  • 13. Mocz G., Helms M. K., Jameson D. M.Gibbons I. R., Biochemistry, 37, 9862 - 9869 (1998), "Probing the Nucleotide Binding Sites of Axonemal Dynein with the Fluorescent Nucleotide Analogue 2'(3')-O-(-N-Methylanthraniloyl)-adenosine 5'-triphosphate"
  • 14. Pechatnikova E.Taylor E. W., J. Biol. Chem., 272, 30735 - 30740 (1997), "Kinetic Mechanism of Monomeric Non-claret Disjunctional Protein (Ncd) ATPase"
  • 15. Bauer C. B., Kuhlmann P. A., Bagshaw C. R.Rayment I., J. Mol. Biol., 274, 394 - 407 (1997), "X-ray Crystal Structure and Solution Fluorescence Characterization of Mg-2'(3')-(N-Methylanthraniloyl) Nucleotides Bound to the Dictyostelium discoideum Myosin Motor Domain"
  • 16. Uyeda K., Wang X. L., Mizuguchi H., Li Y., Nguyen C.Hasemann C. A., J. Biol. Chem., 272, 7867 - 7872 (1997), "The Active Sites of Fructose 6-Phosphate, 2-Kinase-Fructose-2,6-Biphosphatase from Rat Testitits- Roles of ASP-128, THR-52, THR-130, ASN-73, and TYR-197"
  • 17. Moore K. J. M.Lohmann T. M., Biochemistry, 48, 14550 - 14564 (1994), "Kinetic mechanism of Adenine Nucleotide Binding to and Hydrolysis by the Escherichia Coli Rep Monomer .1. Use of Fluorescent Nucleotide Analogues"
  • 18. Woodward S. K. A., Eccleston J. F.Geeves M. A., Biochemistry, 30, 422 - 430 (1991), "Kinetics of the Interaction of 2'(3')-O-(N-Methylanthraniloyl)-ATP with Myosin Subfragment 1 and Actomyosin Subfragment 1: Characterization of Two Acto-S1-ADP Complexes"
  • 19. Hiratsuka T., Biochim. Biophys. Acta, 742, 496 - 508 (1983), "New Ribose-modified Fluorescent Analogs of Adenine and Guanine Nucleotides Available as Substrates for Various Enzymes"