carba-NAD⁺ is a NAD⁺ mimic with improved hydrolytic stability against adenosine diphosphate ribosyl transferase (ART) enzymes and NAD glycohydrolases. carba-NAD⁺ was shown to inhibit activity of the cholera toxin A ART enzyme and NAD⁺-consuming enzymes Sirt3/5, but was ineffective against PARP1 and NAD⁺-glycohydrolase. carba-NAD⁺ is a substrate of NAD⁺-handling enzymes like alcohol dehydrogenases and is suitable as hydrolysis-resistant NAD⁺-mimic for protein crystallizations. Robust cofactor for enzyme-based glucose sensing with glucose dehydrogenases and fluorescence lifetime detection. References: Madern et al., Chembiochem., 21, 2903 - 2907 (2020); von Ketteler et al. Chemphyschem., 13, 1302 - 1306 (2012), Szczepankiewicz et al. J. Org. Chem., 77, 7319 - 7329 (2012).
|Cat. No.||C 403|
|Purity||> 95% HPLC|
|Storage temperature||-20°C / -4°F|
|Molecular weight [g/mol]||661.5 (for free acid)|
|Absorption max [nm]||260|
|Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹]||18000|
|UV pH value||7|