carba-NAD⁺ is a NAD⁺ mimic with improved hydrolytic stability against adenosine diphosphate ribosyl transferase (ART) enzymes and NAD glycohydrolases. carba-NAD⁺ was shown to inhibit activity of the cholera toxin A ART enzyme and NAD⁺-consuming enzymes Sirt3/5, but was ineffective against PARP1 and NAD⁺-glycohydrolase. carba-NAD⁺ is a substrate of NAD⁺-handling enzymes like alcohol dehydrogenases and is suitable as hydrolysis-resistant NAD⁺-mimic for protein crystallizations. Robust cofactor for enzyme-based glucose sensing with glucose dehydrogenases and fluorescence lifetime detection. References: Madern et al., Chembiochem., 21, 2903 - 2907 (2020); von Ketteler et al. Chemphyschem., 13, 1302 - 1306 (2012), Szczepankiewicz et al. J. Org. Chem., 77, 7319 - 7329 (2012).
Cat. No. | C 403 |
CAS number | [112345-60-5] |
Purity | > 95% HPLC |
Salt form | Sodium |
Storage temperature | -20°C / -4°F |
Molecular formula | C₂₂H₂₉N₇O₁₃P₂ |
Molecular weight [g/mol] | 661.5 (for free acid) |
Absorption max [nm] | 260 |
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] | 18000 |
UV pH value | 7 |