0 Items - $0.00
Categories
A to Z

Alternative structural projections:

8-NBD-cAMP

8- (2- [7- Nitro- 4- benzofurazanyl]aminoethylthio)adenosine- 3', 5'- cyclic monophosphate ( 8-NBD-cAMP ), sodium salt
Fluorescent cyclic AMP analogue
Cat. No.: N 002
    
CAS No.: [221905-51-7]
Shipping: ambient
Product Name Price (net) Qty
Cat. No.: N 002-01
Unit: 1 µmol / ~0.6 mg
$174.00
For other salt forms or metabolites please inquire. Potent membrane-permeant fluorescent activator (λexc 471 nm, λem 536 nm) of cAMP-dependent protein kinase. While 8-NBD-cAMP is almost non-fluorescent in aqueous solutions, it fluoresces strongly in hydrophobic environments such as hydrophobic protein binding sites. Detailed technical information available. Reference: Kraemer et al., J. Mol. Biol., 306, 1167 - 1177 (2001); Wu et al., Biophys. J., 100, 1226- 1232 (2011); Chen et. al., Bioorg. Med. Chem. Lett., 22, 4038 - 4043 (2012).
Cat. No. N 002
CAS number [221905-51-7]
Purity > 95% HPLC
Salt form Sodium
Storage temperature -20°C / -4°F
Light-sensitive True
Molecular formula C₁₈H₁₇N₉O₉PS · Na
Molecular weight [g/mol] 589.4
Modifications 8-cAMP
Target PKA (cAK) Activator
Excitation max [nm] 471
Emission max [nm] 536
Lipophilicity 2.24
Absorption max [nm] 463
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 22000
Solvent for UV data MeOH
  • 1. Almahariq M., Tsalkova T., Mei F. C., Chen H., Zhou J., Sastry S. K., Schwede F.Cheng X., Mol. Pharmacol., 83, 122 - 128 (2013), "A Novel EPAC Specific Inhibitor Suppresses Pancreatic Cancer Cell Migration and Invasion"
  • 2. Tsalkova T., Mei F. C.Cheng X., PloS ONE, 7, 0 - 0 (2012), "A Fluorescence-Based High-Throughout Assay for the Discovery of Exchange Protein Directly Activated by cyclic AMP (Epac) Antagonists"
  • 3. Wu S., Vysotskaya Z. V., Xu X., Xie C., Liu Q.Zhou L., Biophys. J., 100, 1226 - 1232 (2011), "State-Dependent cAMP Binding to Functioning HCN Channels Studied by Patch-Clamp Fluorometry"
  • 4. Tsalkova T., Gribenko A. V.Cheng X., Assay Drug Dev. Technol., 9, 88 - 91 (2010), "Exchange Protein Directly Activated by Cyclic AMP Isoform 2 is not a Direct Target of Sulfonylurea Drugs"
  • 5. Tsalkova T., Blumenthal D. K., Mei F. C., White M. A.Cheng X. D., J. Biol. Chem., 284, 23644 - 23651 (2009), "Mechanism of Epac Activation Structural and Functional Analyses of Epac2 Hinge Mutants with Constitutive and Reduced Activities"
  • 6. Schweinsberg S., Moll D., Burghardt N. C., Hahnefeld C., Schwede F., Zimmermann B., Drewianka S., Werner L., Kleinjung F., Genieser H.-G., Schuchhardt J.Herberg F. W., Proteomics, 8, 1212 - 1220 (2008), "Systematic interpretation of cyclic nucleotide binding studies using KinetXBase"
  • 7. Hahnefeld C.Herberg F. W., Abstract of the 13th Protein Kinase Symposium p. 14, 0, 12 - 15 (2002), "cAMP-dependent Protein Kinase: cAMP Analog Screening Using Surface Plasmon Resonance and Fluorescent Methods"
  • 8. Kraemer A., Rehmann H., Cool R. H., Theiss C., de Rooij J., Bos H.Wittinghofer A., J. Mol. Biol., 306, 1167 - 1177 (2001), "Dynamic Interaction of cAMP with the Rap Guanine-nucleotide Exchange Factor Epac1"