8-N3-ε-ATP combines the photosensitive activity of 8-N3-ATP (Cat. No. A 043) and the fluorescent properties of ε-ATP (Cat. No. E 004). It is useful for photoaffinity labelling of ATP binding proteins and subsequent detection of the fluorescent label (λexc 285 nm, λem 412 nm). Alternatively, detection may be achievable by using an ethenoadenosine-specific antibody (1G4, compare: Krebs et al., Anal. Biochem., 314, 108 - 115 (2003)). Detailed technical information available. References: Schäfer et al., Anal. Biochem., 104, 106 - 111 (1980); Schäfer et al., Nucleic Acids Res., 5, 1345 - 1351 (1978).
Vial containing 500 µl of 10 mM aqueous solution of pH 7.6
Cat. No. | A 211 |
CAS number | [66895-05-4] |
Purity | > 95% HPLC |
Salt form | Sodium |
Appearance | 10 mM aqueous solution of pH 7.6 |
Storage temperature | -70°C / -94°F |
Light-sensitive | True |
Molecular formula | C₁₂H₁₅N₈O₁₃P₃ (free acid) |
Molecular weight [g/mol] | 572.2 (free acid) |
Excitation max [nm] | 285 |
Emission max [nm] | 412 |
Absorption max [nm] | 290 |
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] | 10000 |