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5- (3- (2- Azidoacetyl)aminoallyl)- 2'- deoxyuridine- 5'- O- triphosphate ( 5-N₃Ac-AA-dUTP ), sodium salt

Azide-containing analogue of dUTP
Cat. No.: A 358
CAS No.: [pending]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: A 358-0025
Unit: 0.25 µmol / ~152 µg

For other salt forms please inquire. The azide-containing 5-N₃Ac-AA-dUTP is an analogue of dUTP and a suitable molecular tool for the copper(I)-catalysed Huisgen azide-alkyne [3+2] cycloaddition (CuAAC) or copper(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) with strained alkynes, respectively. Both reactions have attracted particular interest for the introduction of reporter groups and for the labelling of various biomolecules like TTP-binding proteins by the so-called click-chemistry technique. 5-Azide-functionalised pyrimidine nucleotides can replace their natural counterpart TTP in polymerase reactions, leading to the generation of a modified and functionalized DNA strand that can subsequently be further reacted and modified using click chemistry methods. References for CuAAC and SPAAC click chemistry: Dommerholt et al., Top. Curr. Chem.374:16 (2016); Presolski et al., Curr. Protoc. Chem. Biol.3, 153 - 162 (2011); Hong et al., Angew. Chem. Int. Ed. Engl., 48, 9879 - 9883 (2009)


Vial containing 250 µl of 1 mM aqueous solution of pH 7.6

Cat. No. A 358
CAS number [pending]
Purity > 95% HPLC
Salt form Sodium
Appearance 1 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Light-sensitive Yes
Molecular formula C₁₄H₂₁N₆O₁₅P₃ (free acid)
Molecular weight [g/mol] 606.3 (free acid)
Absorption max [nm] 290
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 8100
UV pH value 7
Solvent for UV data Water