For other salt forms or a guaranteed higher purity please inquire. 3'-O-Ac-dGTP is an analogue of dGTP with blocked 3’-hydroxy function, which can be used as a transient, pH-sensitive chain terminator during DNA polymerization. The acetyl group of 3'-O-Ac-dGTP is sensitive towards alkaline conditions and can be split off at pH >12.5 within 30 min. to allow further subsequent polymerization steps. References: Rensland et al., Biochemistry, 34, 593 – 599 (1995); Marián, M., Microchem. J., 29, 219 - 227 (1984).
Vial containing 500 µl of 10 mM aqueous solution of pH 7.6.
| Cat. No. | A 278 |
| CAS number | [90290-77-0] |
| Purity | >95% HPLC |
| Salt form | Sodium |
| Appearance | 10 mM aqueous solution |
| Storage temperature | -70°C / -94°F |
| Molecular formula | C₁₂H₁₈N₅O₁₄P₃ (free acid) |
| Molecular weight [g/mol] | 549.2 (free acid) |
| Absorption max [nm] | 13500 |
| Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] | 252 |
| UV pH value | 7 |
| Solvent for UV data | Water |
