The protein kinase G activator cyclic GMP immobilized on agarose by an aminoethylcarbamoyl spacer attached to the ribose 2'-position of the ligand. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins, especially those which tolerate modification of the 2'-ribose hydroxyl group such as some phosphodiesterases. This type of gel is also offered with a longer spacer (2'-AHC-cGMP-Agarose, Cat. No. A 059
) and corresponding PDE-resistant versions (Rp- and Sp-2'-AHC-cGMPS-Agarose, Cat. No's. A 051
& A 052
). Detailed technical information available.Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.