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UTP-γ-AmNS

Uridine- 5'- O- triphosphoro- γ- 1- (5- sulfonic acid)naphthylamidate ( UTP-γ-AmNS ), sodium salt
Cat. No.: U 014
    
CAS No.: [72218-69-0]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: U 014-05
Unit: 5 µmol / ~3.4 mg
$375.00

For other salt forms or a guaranteed higher purity please inquire. Fluorescent analogue of UTP useful for an assay of enzymes specialized to cleave α/β-phosphodiester bonds. Detailed technical information available. Reference: Yarbrough & Bock, J. Biol. Chem., 255, 9907 - 9911 (1980).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6.

Cat. No. U 014
CAS number [72218-69-0]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₁₉H₂₂N₃O₁₇P₃S (free acid)
Molecular weight [g/mol] 689,4 (free acid)
Absorption max [nm] 246
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 31000
  • 1. Bhat J., Rane R., Solapure S. M., Sarkar D., Sharma U., Harish M. N., Lamb S., Plant D., Alcock P., Peters S., Barde S.Roy R. K., J. Biomol Screen., 11, 968 - 976 (2006), "High-Throughput Screening of RNA Polymerase Inhibitors Using a Fluorescent UTP Analog"
  • 2. Wu F. Y.-H.Tyagi S. C., J. Biol. Chem., 262, 13147 - 13154 (1987), "Fluoresence Resonance Energy Transfer Studies on the Proximity Relationship between the Intrinsic Metal Ion and Substrate Binding Sites of Escherichia coli RNA polymerase"
  • 3. Pollack S. E.Auld D. S., Anal. Biochem., 127, 81 - 88 (1982), "Fluorescent Nucleotide Triphosphate Substrates for Snake Venom Phosphodiesterase"
  • 4. Yarbrough L. R.Bock J. L., J. Biol. Chem., 255, 9907 - 9911 (1980), "Stacking Interactions in Fluorescent Nucleotide Analogs Containing 1-Aminonaphthalene-5-Sulfonate at the Phosphoryl Terminus"
  • 5. Yarbrough L. R., Schlageck J. G.Baughman M., J. Biol. Chem., 254, 12069 - 12073 (1979), "Synthesis and Properties of Fluorescent Nucleotide Substrates for DNA-dependent RNA Polymerases"
  • 6. Schlageck J. G., Baughman M.Yarbrough L. R., J. Biol. Chem., 254, 12074 - 12077 (1979), "Spectroscopic techniques for Study of Phosphodiester Bond Formation by Escherichia coli RNA Polymerase"
  • 7. Yarbrough L. R., Biochem. Biophys. Res. Commun., 81, 35 - 41 (1978), "Synthesis and Properties of a New Fluorescent Analog of ATP: Adenosine-5'-Triphosphoro-Gamma-1-(5-Sulfonic Acid) Napthylamidate"