The PDE-resistant PKG activator Sp-cGMPS
(Cat. No. G 017) immobilized on agarose by an aminohexylcarbamoyl spacer attached to the ribose 2'-position of the ligand. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins, especially those which tolerate modification of the 2'-ribose hydroxyl group such as some phosphodiesterases. This type of gel is also offered with normal cGMP (2'-AHC-cGMP-Agarose
, Cat. No. A 059). Detailed technical information available. Compare: Corrie et al., Prot. Expr. Purif.,
3, 417 - 420 (1992).Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.