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Sp-2-AHA-cAMPS-Agarose

2- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer; immobilized on agarose gel ( Sp-2-AHA-cAMPS-Agarose )
PDE-resistant Sp-cAMPS immobilized on agarose gel 
Cat. No.: A 069
    
CAS No.: [pending]
Shipping: ambient
Product Name Price (net) Qty
Cat. No.: A 069-06
Unit: 0.6 ml
$314.00
Cat. No.: A 069-25
Unit: 2.5 ml
$980.00
Cat. No.: A 069-60
Unit: 6 ml
$1,975.00
The protein kinase A activator Sp-cAMPS (Cat. No. A 003) immobilized on agarose by an aminohexylamino spacer attached to position 2 of the ligand. Free Sp-cAMPS is only very slowly metabolized by mammalian phosphodiesterases and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase A compared to cAMP results in milder desorption conditions during affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This ligand is offered with a shorter spacer (Sp-2-AEA-cAMPS-Agarose, Cat. No. A 087) as well. Detailed technical information available. References: Bertinetti et al., BMC Chem Biol, 9 (2009); Hanke et al., N. Biotechnol., 28, 294 - 301 (2011).
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Cat. No. A 069
CAS number [pending]
Storage temperature 4°C / 39°F
cAMP modified at the exocyclic oxygen axial (Sp-)
Modifications 2-cAMP
  • 1. Hanke S. E., Bertinetti D., Badel A., Schweinsberg S., Genieser H.-G.Herberg F. W., N. Biotechnol., 28, 294 - 301 (2011), "Cyclic Nucleotides as Affinity Tools: Phosphorothioate cAMP Analogues Adress Specific PKA Subproteomes"
  • 2. Bertinetti D., Schweinsberg S., Hanke S. E., Schwede F., Bertinetti O., Drewianka S., Genieser H.-G.Herberg F. W., BMC Chem Biol, 9, 0 - 0 (2009), "Chemical tools selectively target components of the PKA system"