The protein kinase A activator Sp-cAMPS
(Cat. No. A 003) immobilized on agarose by an aminohexylamino spacer attached to position 2 of the ligand. Free Sp-cAMPS is only very slowly metabolized by mammalian phosphodiesterases and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase A compared to cAMP results in milder desorption conditions during affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This ligand is offered with a shorter spacer (Sp-2-AEA-cAMPS-Agarose
, Cat. No. A 087) as well. Detailed technical information available. References: Bertinetti et al., BMC Chem Biol
(2009); Hanke et al., N. Biotechnol
, 294 - 301 (2011).Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.