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Rp-dATP-α-S

2'- Deoxyadenosine- 5'- O- (1- thiotriphosphate), Rp isomer (Rp-dATP-α-S), sodium salt
Cat. No.: D 006
    
CAS No.: [87358-15-4]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: D 006-05
Unit: 5 µmol / ~2.5 mg
$224.00

For other salt forms or a guaranteed higher purity please inquire. Useful for modulation of dATP-responsive receptors and determination of their stereospecificity. Increased metabolic stability compared to dATP. Not accepted by DNA polymerase. Detailed technical information available. Reference: Romaniuk et al., J. Biol. Chem., 257, 7684 - 7688 (1982).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6

Cat. No. D 006
CAS number [87358-15-4]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Molecular formula C₁₀H₁₆N₅O₁₁P₃S (free acid)
Molecular weight [g/mol] 507,3 (free acid)
Absorption max [nm] 259
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 15200
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  • 2. Ryder S. P.Strobel S. A., Methods Enzymol., 18, 38 - 50 (1999), "Nucleotide Analog Interference Mapping"
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  • 8. Lazewska D.Guranowski A., Nucleic Acids Res., 18, 6083 - 6088 (1990), "P-alpha- chiral Phosphorothioate Analogues of Bis (5'- adenosyl)tetraphosphate (Ap4A~#~ their Enzymatic Synthesis and Degradation"
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  • 12. Goody R., Isakov M.Labeit S., Ger. Offen. DE 3374, 546, 0 - 0 (1985), "DNA Sequencing Involving Alpha-thiodeoxyribonucleotide triphosphate-labeled DNA and Exonuclease Digestion, Synthesis of Alpha-thionucleotide triphosphates and Resolution of the Resulting Diastereomeric Mixture"
  • 13. Eckstein F., Angew. Chemie, 95, 431 - 447 (1983), "Phosphorothioatanaloga von Nukleotiden - Werkzeuge zur Untersuchung biochemischer Prozesse"
  • 14. Marlier J. F.Benkovic S. J., Biochemistry, 21, 2349 - 2356 (1982), "On the Mechanism of de Novo Polymerization by Form I Polynucleotide Phosphorylase of Micrococcus luteus"
  • 15. Romaniuk P. J.Eckstein F., J. Biol. Chem., 257, 7684 - 7688 (1982), "A Study of the Mechanism of T4 DNA Polymerase with Diastereomeric Phosphorothioate Analogues of Deoxyadenosine Triphosphate"
  • 16. Gupta A., DeBrosse C.Benkovic S. J., J. Biol. Chem., 257, 7689 - 7692 (1982), "Template-Primer-dependent Turnover of (Sp)-dATP@S by T4 DNA Polymerase"
  • 17. Lowe G., Tansley G.Cullis P. M., J. Chem. Soc. Chem. Commun., 595, 0 - 0 (1982), "Synthesis of Adenosine 5' [(R)alpha-17O]Triphosphate"
  • 18. Frey P. A., Tetrahedron, 38, 1541 - 1567 (1982), "Stereochemistry of Enzymatic Reactions of Phosphates"
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