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Rp-8-AHA-cAMPS-Agarose

8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer; immobilized on agarose gel ( Rp-8-AHA-cAMPS-Agarose )
cyclic AMP antagonist immobilized on agarose gel
Cat. No.: A 012
    
CAS No.: [pending]
Shipping: ambient
Product Name Price (net) Qty
Cat. No.: A 012-06
Unit: 0.6 ml
$248.00
Cat. No.: A 012-25
Unit: 2.5 ml
$774.00
Cat. No.: A 012-60
Unit: 6 ml
$1,559.00
The metabolically stable protein kinase A inhibitor Rp-cAMPS (Cat. No. A 002) immobilized on agarose by an aminohexylamino spacer attached to position 8 of the ligand. Free Rp-cAMPS binds to the protein kinase A holoenzyme but prevents dissociation of catalytic and regulatory subunits. In addition it is not hydrolized by mammalian phosphodiesterases. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinase A (presumably as its holoenzyme), phosphodiesterases and others. This ligand is offered with a shorter spacer (Rp-8-AEA-cAMPS-Agarose, Cat. No. A 007) as well. Detailed technical information available. References: Margarucci et al., Mol. BioSyst., 7, 2311 - 2319 (2011); Bertinetti et al., BMC Chem Biol, 9 (2009).Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Cat. No. A 012
CAS number [pending]
Storage temperature 4°C / 39°F
cAMP modified at the exocyclic oxygen equatorial (Rp-)
Modifications 8-cAMP
  • 1. Hanke S. E., Bertinetti D., Badel A., Schweinsberg S., Genieser H.-G.Herberg F. W., N. Biotechnol., 28, 294 - 301 (2011), "Cyclic Nucleotides as Affinity Tools: Phosphorothioate cAMP Analogues Adress Specific PKA Subproteomes"
  • 2. Margarucci L., Roest M., Preisinger C., Bleijerveld O. B., van Holten T. C., Heck A. J. R.Scholten A., Mol. Biosyst., 7, 2311 - 2319 (2011), "Collagen Stimulation of Platelets Induces a Rapid Spatial Response of cAMP and cGMP Signaling Scaffolds"
  • 3. Bertinetti D., Schweinsberg S., Hanke S. E., Schwede F., Bertinetti O., Drewianka S., Genieser H.-G.Herberg F. W., BMC Chem Biol, 9, 0 - 0 (2009), "Chemical tools selectively target components of the PKA system"
  • 4. Stokka A. J., Gesellchen F., Carlson C. R., Scott J. D., Herberg F. W.Tasken K., Biochem. J., 400, 493 - 499 (2006), "Characterization of A-kinase-anchoring Disruptors Using a Solution-based Assay"