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Rp-8-AEA-cAMPS-Agarose

8- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose gel ( Rp-8-AEA-cAMPS-Agarose )
Immobilized cAMP Antagonist
Cat. No.: A 007
    
CAS No.: [pending]
Shipping: ambient
Product Name Price (net) Qty
Cat. No.: A 007-06
Unit: 0.6 ml
$248.00
Cat. No.: A 007-25
Unit: 2.5 ml
$774.00
Cat. No.: A 007-60
Unit: 6 ml
$1,559.00
The metabolically stable protein kinase A inhibitor Rp-cAMPS (Cat. No. A 002) immobilized on agarose by an aminoethylamino spacer attached to position 8 of the ligand. Free Rp-cAMPS binds to the protein kinase A holoenzyme, but prevents dissociation to free catalytic and regulatory subunits. In addition, it is not hydrolized by mammalian phosphodiesterases. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinase A (presumably as its holoenzyme), phosphodiesterases and others. This ligand is offered with a longer spacer (Rp-8-AHA-cAMPS-Agarose, Cat. No. A 012) as well. Detailed technical information available.
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Cat. No. A 007
CAS number [pending]
Storage temperature 4°C / 39°F
cAMP modified at the exocyclic oxygen equatorial (Rp-)
Modifications 8-cAMP
  • 1. Hanke S. E., Bertinetti D., Badel A., Schweinsberg S., Genieser H.-G.Herberg F. W., N. Biotechnol., 28, 294 - 301 (2011), "Cyclic Nucleotides as Affinity Tools: Phosphorothioate cAMP Analogues Adress Specific PKA Subproteomes"
  • 2. Iwitzki F., van Bemmelen M. X. P., Genieser H.-G.Jastorff B., Proc. Intl. Conf. Second Messengers Phosphoprot. UK, 0, 0 - 0 (1992), "New Affinity Materials for Cyclic AMP Binding Proteins"
  • 3. Iwitzki F., van Bemmelen M. X. P., Genieser H.-G.Jastorff B., 3rd Swed.- Germ. Workshop Nucleic Acid Synth. Struct. Funct. Sweden, 0, 0 - 0 (1992), "cAMP Analogues Designed for Affinity Chromatography"
  • 4. Dills W. L., Beavo J. A., Bechtel P. J.Krebs E. G., Biochem. Biophys. Res. Commun., 62, 70 - 77 (1975), "Purification of Rabbit Skeletal Muscle Protein Kinase Regulatory Subunit Using Cyclic Adenosine- 3': 5'- Monophosphate Affinity Chromatography"