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PO₄-AM₃

Phosphate tris(acetoxymethyl)ester ( PO₄-AM₃ )
Control reagent for nucleotide-AM ester applications
Cat. No.: P 030
    
CAS No.: [148334-61-6]
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Product Name Price (net) Qty
Cat. No.: P 030-003
Unit: 0.33 µmol / ~0.1 mg
$60.00

Membrane-permeant prodrug of inorganic phosphate. PO4-AM3 is recommended as control reagent in nucleotide-AM ester applications to test for side effects of acetic acid and formaldehyde. After permeation and intracellular metabolic activation by endogenous esterases the highly polar inorganic phosphate (PO43-) is released. In addition, the AM ester groups liberate acetic acid and formaldehyde, two metabolites with potential biological functions. References: Schultz et al., J. Biol. Chem., 268, 6316 - 6322 (1993); Chepurny et al., J. Biol. Chem., 284, 10728 - 10736 (2009).

 

Please note: 0.33 μmol of PO4-AM3 is stoichiometrically equivalent to 1 μmol of a cyclic nucleotide AM ester. 

Cat. No. P 030
CAS number [148334-61-6]
Purity > 95% (MS)
Storage temperature -20°C / -4°F
Molecular formula C₉H₁₅O₁₀P
Molecular weight [g/mol] 314.2
  • 1. Wolter S., Kloth C., Golombek M., Dittmar F., Försterling L.Seifert R., Biochem. Pharmacol., 98, 119 - 131 (2015), "cCMP Causes Caspase-dependent Apotosis in Mouse Lymphoma Cell Lines"
  • 2. Henquin J.-C.Nenquin M., Endocrinol., 0, 0 - 0 (2014), "Activators of PKA and Epac Distinctly Influence Insulin Secretion and Cytosolic Ca2+ in Female Mouse Islets Stimulated by Glucose and Tolbutamide"
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  • 4. Chepurny O. G., Bertinetti D., Diskar M., Leech C. A., Afshari P., Tsalkova T., Cheng X., Schwede F., Genieser H.-G., Herberg F. W.Holz G. G., Mol. Endocrinol., 27, 1267 - 1282 (2013), "Stimulation of Proglucagon Gene Expression by Human GPR119 in Enteroendocrine L-cell Line GLUTag"
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  • 6. Chepurny O. G., Leech C. A., Kelley G. G., Dzhura I., Dzhura E., Li X., Rindler M. J., Schwede F., Genieser H.-G.Holz G. G., J. Biol. Chem., 284, 10728 - 10736 (2009), "Enhanced Rap1 activation and insulin secretagogue properties of an acetoxymethyl ester of an Epac-selective cyclic AMP analog in rat INS-1 cells: Studies with 8-pCPT-2'-O-Me-cAMP-AM."
  • 7. Kelley G. G., Chepurny O. G., Schwede F., Genieser H.-G., Leech C. A., Roe M. W., Li X., Dzhura I., Dzhura E., Afshari P.Holz G. G., Islets, 1, 260 - 265 (2009), "Glucose-dependent Potentation of Mouse Islet Insulin Secretion by Epac Activator 8-pCPT-2'-O-Me-cAMP-AM"
  • 8. Schultz C., Vajanaphanich M., Harootunian A. T., Sammak P. J., Barrett K. E.Tsien R. Y., J. Biol. Chem., 268, 6316 - 6322 (1993), "Acetoxymethyl Esters of Phosphates: Enhancement of the Permeability and Potency of cAMP"