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ε-NAD⁺

β- Nicotinamide- 1, N⁶- ethenoadenine dinucleotide ( ε-NAD⁺ / 1,N6-etheno-NAD+ ), sodium salt

Cat. No.: N 010
    
CAS No.: [38806-38-1]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: N 010-10
Unit: 10 µmol / ~6.9 mg
$169.00
For other salt forms or analogues please inquire. Fluorescent analogue of ß-NAD+ ( λexc 300 nm, λem 410 nm) that can be used to analyse ADP-ribosylation, for fluorometric assays and for preparation of fluorescent poly-ADP-ribose. Detailed technical information and references available. References: Favilla & Mazzini, Biochim. Biophys. Acta, 788, 48 - 57 (1984); Klebl & Pette, Anal. Biochem., 239, 145 - 152 (1996); Shirato et al., Biochem. Biophys. Res. Commun., 355, 451 - 456 (2007).
Cat. No. N 010
CAS number [38806-38-1]
Purity > 95% HPLC
Salt form Sodium
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₂₃H₂₇N₇O₁₄P₂ (free acid)
Molecular weight [g/mol] 687.5 (free acid)
Excitation max [nm] 300
Emission max [nm] 410
Absorption max [nm] 265
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 10200
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  • 4. Shirato M., Tozawa S., Maeda D., Watanabe M., Nakagama H.Masutani M., Biochem. Biophys. Res. Commun., 355, 451 - 456 (2007), "Poly(Etheno ADP-Ribose) Blocks Poly(ADP-Ribose) Glycohydrolase Activity"
  • 5. Song E.-K., Park H.-J., Kim J.-S., Lee H.-H., Kim U.-H.Han M.-K., J. Biochem. Biophys. Methods, 63, 161 - 169 (2005), "A Novel Fluorometric Assay for ADP-ribose Pyrophosphatase Activity"
  • 6. Krebs C., Koestner W., Nissen M., Welge V., Parusel I., Malavasi F., Leiter E. H., Santella R. M., Haag F.Koch-Nolte F., Anal. Biochem., 314, 108 - 115 (2003), "Flow Cytometric and Immunoblot Assays for Cell Surface ADP-Ribosylation Using a Monoclonal Antibody Specific for Ethenoadenosine"
  • 7. Schweitzer K., Mayr G. W.Guse A. H., Anal. Biochem., 299, 218 - 226 (2001), "Assay for ADP-Ribosyl Cyclase by Reverse-Phase High-Performance Liquid Chromatography"
  • 8. Davis R. E., Mysore V., Browning J. C., Hsieh J. C., Lu Q.-A. T.Katsikis p. D., J. Histochem. Cytochem., 46, 1279 - 1289 (1998), "In Situ Staining for Poly(ADP-Ribose) Polymerase Activity Using an NAD Analogue"
  • 9. Basso L. A., Engel P. C.Walmsley A. R., Biochim. Biophys. Acta, 1340, 63 - 71 (1997), "Kinetic Studies on The Binding of 1,N6-Etheno-NAD+ to Glutamate Dehydrogenase from Clostridium Symbiosum"
  • 10. Klebl B. M.Pette D., Anal. Biochem., 239, 145 - 152 (1996), "A Fluorometric Assay for Measurement of Mono-ADP-Ribosyltransferase Activity"
  • 11. Favilla R.Mazzini A., Biochim. Biophys. Acta, 788, 48 - 57 (1984), "The Binding of 1,N6-Etheno-NAD to Bovine Liver Glutamate Dehydrogenase"
  • 12. Barrio J. R., Secrist J. A.Leonard N. J., Proc. Nat. Acad. Sci. USA, 69, 2039 - 2042 (1972), "A Fluorescent Analog of Nicotinamide Adenine Dinucleotide"