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MANT-dATP

2'- Deoxy- 3'- O- (N'- methylanthraniloyl)adenosine- 5'- O- triphosphate ( MANT-dATP )
Fluorescent analogue of 2'-deoxy-ATP
Cat. No.: D 085
    
CAS No.: [128113-53-1]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: D 085-05
Unit: 5 µmol / ~3.1 mg
$132.00

For other salt forms please inquire.
Fluorescent analogue of 2'-deoxyadenosine-5’-triphosphate (λexc 350 nm; λem 446 nm), useful for research into dATP-dependent receptor proteins. The MANT fluorophore has a certain sensitivity for its environment and can change its spectral properties upon binding (compare: Hiratsuka, Biochim. Biophys. Acta, 742, 496 - 508 (1983)). Detailed technical information available. Reference: Nomanbhoy & Schimmel, Bioorg. Med. Chem. Lett., 11, 1485 - 1491 (2001).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6

Cat. No. D 085
CAS number [128113-53-1]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₁₈H₂₃N₆O₁₃P₃ (free acid)
Molecular weight [g/mol] 624,3 (free acid)
Excitation max [nm] 350
Emission max [nm] 446
Absorption max [nm] 255
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 23300
UV pH value 8
  • 1. Seifert R.Dove S., Pharmacol. Therapeut., 140, 200 - 212 (2013), "Inhibitors of Bacillus anthracis Edema Factor"
  • 2. Wang J. L., Guo J.-X., Zhang Q.-Y., Wu J. J.-Q., Seifert R.Lushington G. H., Bioorg. Med. Chem., 15, 2993 - 3002 (2007), "A Conformational Transition in the Adenylyl Cyclase Catalytic Site Yields Different Binding Modes for Ribosyl-modified and Unmodified Nucleotide Inhibitors"
  • 3. Nomanbhoy T. K.Schimmel P., Bioorg. Med. Chem. Lett., 11, 1485 - 1491 (2001), "Active Site of an Aminoacyl-tRNA Synthetase Dissected by Energy-Transfer-Dependent Fluorescence"
  • 4. Ni D. Q., Shaffer J.Adams J. A., Protein Sci., 9, 1818 - 1827 (2000), "Insights into Nucleotide Binding in Protein Kinase A Using Fluorescent Adenosine Derivatives"
  • 5. Woodward S. K. A., Eccleston J. F.Geeves M. A., Biochemistry, 30, 422 - 430 (1991), "Kinetics of the Interaction of 2'(3')-O-(N-Methylanthraniloyl)-ATP with Myosin Subfragment 1 and Actomyosin Subfragment 1: Characterization of Two Acto-S1-ADP Complexes"