For other salt forms or a guaranteed higher purity please inquire. ATP-γ-AmNS is a fluorescent analogue of ATP with λem 460 nm (quantum yield = 0.63). Upon cleavage of the α-β-phosphoryl bond the emission maximum shifts to 475 nm. Detailed technical information available. References: Yarbrough, Biochem. Biophys. Res. Comm., 81, 35 - 41 (1978); Yarbrough & Bock, J. Biol. Chem., 255, 9907 - 9911 (1980).
Vial containing 500 µl of 10 mM aqueous solution of pH 7.6.
| Cat. No. | A 176 |
| CAS number | [66492-86-2] |
| Purity | > 95% HPLC |
| Salt form | Sodium |
| Appearance | 10 mM aqueous solution of pH 7.6 |
| Storage temperature | -70°C / -94°F |
| Light-sensitive | True |
| Molecular formula | C₂₀H₂₃N₆O₁₅P₃S (free acid) |
| Molecular weight [g/mol] | 712.4 (free acid) |
| Emission max [nm] | 460 |
| Absorption max [nm] | 246 |
| Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] | 31000 |
- 1. Wu F. Y.-H.Tyagi S. C., J. Biol. Chem., 262, 13147 - 13154 (1987), "Fluoresence Resonance Energy Transfer Studies on the Proximity Relationship between the Intrinsic Metal Ion and Substrate Binding Sites of Escherichia coli RNA polymerase"
- 2. Pollack S. E.Auld D. S., Anal. Biochem., 127, 81 - 88 (1982), "Fluorescent Nucleotide Triphosphate Substrates for Snake Venom Phosphodiesterase"
- 3. Yarbrough L. R.Bock J. L., J. Biol. Chem., 255, 9907 - 9911 (1980), "Stacking Interactions in Fluorescent Nucleotide Analogs Containing 1-Aminonaphthalene-5-Sulfonate at the Phosphoryl Terminus"
- 4. Yarbrough L. R., Schlageck J. G.Baughman M., J. Biol. Chem., 254, 12069 - 12073 (1979), "Synthesis and Properties of Fluorescent Nucleotide Substrates for DNA-dependent RNA Polymerases"
- 5. Schlageck J. G., Baughman M.Yarbrough L. R., J. Biol. Chem., 254, 12074 - 12077 (1979), "Spectroscopic techniques for Study of Phosphodiester Bond Formation by Escherichia coli RNA Polymerase"
- 6. Yarbrough L. R., Biochem. Biophys. Res. Commun., 81, 35 - 41 (1978), "Synthesis and Properties of a New Fluorescent Analog of ATP: Adenosine-5'-Triphosphoro-Gamma-1-(5-Sulfonic Acid) Napthylamidate"
