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Alternative structural projections:

8-AEA-cAMP-Agarose

8- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel
Cat. No.: A 020
    
CAS No.: [pending]
Shipping: ambient
Product Name Price (net) Qty
Cat. No.: A 020-06
Unit: 0.6 ml
$119.00
Cat. No.: A 020-25
Unit: 2.5 ml
$377.00
Cat. No.: A 020-60
Unit: 6 ml
$757.00

The second messenger cyclic AMP immobilized on agarose by an aminoethylamino spacer attached to position 8 of the ligand. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This ligand is also offered with a longer spacer (8-AHA-cAMP-Agarose, Cat. No. A 028) and as a PDE-resistant version (Sp-8-AEA-cAMPS-Agarose, Cat. No. A 008). Detailed technical information available. References: Dills et al., Biochemistry, 15, 3724 - 3730 (1976); Scholten et al., J. Proteome Res., 5, 1435 - 1447 (2006).

Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.

 

Appearance: Suspension in 30 mM Na2HPO4 buffer (pH 7).

Cat. No. A 020
CAS number [pending]
Appearance Suspension in 30 mM Na₂HPO₄ buffer (pH 7)
Storage temperature 4°C / 39°F
Modifications 8-cAMP
  • 1. Donaldson L.Meier S., Methods Mol. Biol., 1016, 155 - 173 (2013), "An Affinity Pull-Down Approach to Identify the Plant Cyclic Nucleotide Interactome"
  • 2. Hanke S. E., Bertinetti D., Badel A., Schweinsberg S., Genieser H.-G.Herberg F. W., N. Biotechnol., 28, 294 - 301 (2011), "Cyclic Nucleotides as Affinity Tools: Phosphorothioate cAMP Analogues Adress Specific PKA Subproteomes"
  • 3. Bertinetti D., Schweinsberg S., Hanke S. E., Schwede F., Bertinetti O., Drewianka S., Genieser H.-G.Herberg F. W., BMC Chem Biol, 9, 0 - 0 (2009), "Chemical tools selectively target components of the PKA system"
  • 4. Scholten A., van Veen T. A. B., Vos M.Heck A. J. R., J. Proteome Res., 6, 1705 - 1717 (2007), "Diversity of cAMP-Dependent Protein Kinase Isoforms and Their Anchoring Proteins in Mouse Ventricular Tissue"
  • 5. Scholten A., Poh M. K., Vandekerckhove J., van Breukelen B., Vos M. A.Heck A. J. R., J. Proteome Res., 5, 1435 - 1447 (2006), "Analysis of the cGMP/cAMP Interactome Using a Chemical Proteomics Approach in Mammalian Heart Tissue Validates Sphingosine Kinase Type 1-interacting Protein as a Genuine and Highly Abundant AKAP"
  • 6. Honda A., Adams S. R., Sawyer C. L., Lev-Ram V., Tsien R. Y.Dostmann W. R. G., PNAS, 98, 2437 - 2442 (2001), "Spatiotemporal Dynamics of Guanosine 3'5'-cyclic -Monophosphate Revealed by a Genetically Encoded, Fluorescent Indicator"
  • 7. Pöhler D., Butt E., Meißner J., Müller S., Walter U., Lohmann S. M.Jarchau T., FEBS Lett., 374, 419 - 425 (1995), "Expression, Purification, and Characterization of the cGMP-dependent Protein Kinases Iß and II Using the Baculovirus System"
  • 8. Rannels S. R.Corbin J. D., J. Biol. Chem., 255, 7085 - 7088 (1980), "Two Different Intrachain cAMP Binding Sites of cAMP-Dependent Protein Kinases"
  • 9. Dills W. L., Beavo J. A., Bechtel P. J., Myers K. R., Sakai L. J.Krebs E. G., Biochemistry, 15, 3724 - 3731 (1976), "Binding of Adenosine 3', 5'- Monophosphate Dependent Protein Kinase Regulatory Subunit to Immobilized Cyclic Nucleotide Derivatives"