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ε-ATP

1, N⁶- Ethenoadenosine- 5'- O- triphosphate ( ε-ATP ), sodium salt
Fluorescent analogue of ATP
Cat. No.: E 004
    
CAS No.: [60777-99-3]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: E 004-05
Unit: 5 µmol / ~2.7 mg
$80.00

For other salt forms or a guaranteed higher purity please inquire. Fluorescent ATP analogue; λexc 300 nm, λem 415 nm. Detailed technical information available. References: Shahak et al., FEBS Lett., 33, 293 - 296 (1973); Leonard, N.J., CRC Crit. Reviews Biochem., 15, 125 - 199 (1983); Kinoshita, Y.; Nishigaki, K., J. Biochem., 122, 205 - 211 (1997). 

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6

Cat. No. E 004
CAS number [60777-99-3]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₁₂H₁₆N₅O₁₃P₃ (free acid)
Molecular weight [g/mol] 531,2 (free acid)
Excitation max [nm] 300
Emission max [nm] 415
Absorption max [nm] 275
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 6000
  • 1. Bobalova J., Bobal P.Mutafova-Yambolieva V. N., Anal. Biochem., 305, 269 - 276 (2002), "High-Performance Liquid Chromatographic Technique for Detection of a Fluorescent Analogue of ADP-Ribose in Isolated Blood Vessel Preparation"
  • 2. Kinoshita Y.Nishigaki K., J. Biochem., 122, 205 - 211 (1997), "Unexpectedly General Replaceability of ATP in ATP-Requiring Enzymes"
  • 3. Chabbert M., Lami H.Williamson J., J. Biol. Chem., 266, 5395 - 5400 (1991), "Cofactor-induced Orientation of the DNA Bases in Single-stranded DNA Complexed with RecA Protein. A Fluorescence Anisotropy and Time-decay Study"
  • 4. Kwiatkowski A. P.King M. M., Biochemistry, 26, 7636 - 7640 (1987), "Mapping of the Adenosine 5'-Triphosphate Binding Site of Type II Calmodulin-Dependent Protein Kinase"
  • 5. Leonard N. J., CRC Crit. Reviews Biochem., 15, 125 - 199 (1983), "Etheno-substituted Nucleotides and Coenzymes: Fluorescence and Biological Activity"
  • 6. Dorgan L. J.Schuster S. M., Arch. Biochem. Biophys., 207, 165 - 174 (1981), "The Interaction of Nucleotides and Yeast Hexokinase"
  • 7. Shahak Y., Chipman D. M.Shavit N., FEBS Lett., 33, 293 - 296 (1973), "Photophosphorylation Studies with Fluorescent Adenine Nucleotide Analogs"