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8-N₃-ε-ATP

8- Azido- 1, N⁶- ethenoadenosine- 5'- O- triphosphate ( 8-N₃-ε-ATP ), sodium salt
Cat. No.: A 211
    
CAS No.: [66895-05-4]
Shipping: Dry ice
Product Name Price (net) Qty
Cat. No.: A 211-05
Unit: 5 µmol / ~2.9 mg
$315.00

8-N3-ε-ATP combines the photosensitive activity of 8-N3-ATP (Cat. No. A 043) and the fluorescent properties of ε-ATP (Cat. No. E 004). It is useful for photoaffinity labelling of ATP binding proteins and subsequent detection of the fluorescent label (λexc 285 nm, λem 412 nm). Alternatively, detection may be achievable by using an ethenoadenosine-specific antibody (1G4, compare: Krebs et al., Anal. Biochem., 314, 108 - 115 (2003)). Detailed technical information available. References: Schäfer et al., Anal. Biochem., 104, 106 - 111 (1980); Schäfer et al., Nucleic Acids Res., 5, 1345 - 1351 (1978).

 

Vial containing 500 µl of 10 mM aqueous solution of pH 7.6

Cat. No. A 211
CAS number [66895-05-4]
Purity > 95% HPLC
Salt form Sodium
Appearance 10 mM aqueous solution of pH 7.6
Storage temperature -70°C / -94°F
Light-sensitive True
Molecular formula C₁₂H₁₅N₈O₁₃P₃ (free acid)
Molecular weight [g/mol] 572,2 (free acid)
Excitation max [nm] 285
Emission max [nm] 412
Absorption max [nm] 290
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] 10000
  • 1. Schäfer H.-J., Scheurich P., Rathgeber G.Dose K., Anal. Biochem., 104, 106 - 111 (1980), "Fluorescent Photoaffinity Labeling of F1 ATPase from Micrococcus Luteus with 8-Azido-1,N6-Etheno-Adenosine 5'-Triphosphate"
  • 2. Schäfer H.-J., Scheurich P., Rathgeber G.Dose K., Nucleic Acids Res., 5, 1345 - 1351 (1978), "Synthesis and Properties of 8-azido-1, N6-etheno Adenosine Triphosphate - a Fluorescent and Photosensitive ATP Analog"