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8-[Fluo]-cAMP

8- (2- [Fluoresceinyl]aminoethylthio)adenosine- 3', 5'- cyclic monophosphate ( 8-[Fluo]-cAMP / 8-[Fluo]-AET-cAMP ), sodium salt
cyclic AMP conjugated with fluorescein
Cat. No.: F 002
    
CAS No.: [293296-57-8]
Shipping: ambient
Product Name Price (net) Qty
Cat. No.: F 002-01
Unit: 1 µmol / ~0.8 mg
$138.00

For other salt forms or metabolites please inquire. Fluorescent cAMP analogue (λexc 494 nm, λem 517 nm). Detailed technical information available. Reference: Schwede et al., Biochemistry, 39, 8803 - 8812 (2000); Wu et al., Biophys. J., 100, 1226 - 1232 (2011); Lolicato et al., J. Biol. Chem., 286, 44811 - 44820 (2011)

Cat. No. F 002
CAS number [293296-57-8]
Purity > 95% HPLC
Salt form Sodium
Storage temperature -20°C / -4°F
Light-sensitive True
Molecular formula C₃₃H₂₇N₇O₁₁PS₂ · Na
Molecular weight [g/mol] 815.7
Modifications 8-cAMP
Target PKA (cAK) Activator
Excitation max [nm] 494
Emission max [nm] 517
Lipophilicity 2.49
Absorption max [nm] ~494
Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹] ~79000
UV pH value 9
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  • 2. Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F. W., Bolognes M., Martin H., Fasolini M., Bertrand J. A., Arrigoni C., Thiel G.Moroni A., J. Biol. Chem., 286, 44811 - 44820 (2011), "Tetramerization Dynamics of C-terminal Domain Underlies Isoform-specific cAMP Gating in Hyperpolarization-activated Cyclic Nucleotide-gated Channels"
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  • 11. Hahnefeld C.Herberg F. W., Abstract of the 13th Protein Kinase Symposium p. 14, 0, 12 - 15 (2002), "cAMP-dependent Protein Kinase: cAMP Analog Screening Using Surface Plasmon Resonance and Fluorescent Methods"
  • 12. Schwede F., Christensen A., Liauw S., Hippe T., Kopperud R. K., Jastorff B.Doeskeland S. O., Biochemistry, 39, 8803 - 8812 (2000), "8-Substituted cAMP Analogues Reveal Marked Differences in Adaptability, Hydrogen Bonding, and Charge Accomodation between Homologous Binding Sites (AI/AII and BI/BII) in cAMP Kinase I and II"
  • 13. Mucignat-Caretta C., Conti V.Caretta A., Neurosci. Lett., 273, 1 - 4 (1999), "cAMP-dependent Protein Kinase Type R I is Found in Clusters in the Rat Detergent-insoluble Neuronal Fraction"
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