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Alternative structural projections:

8-AET-cGMP-Agarose

8- (2- Aminoethylthio)guanosine- 3', 5'- cyclic monophosphate; immobilized on agarose gel ( 8-AET-cGMP-Agarose )
cyclic GMP immobilized on agarose gel 
Cat. No.: A 019
    
CAS No.: [pending]
Shipping: ambient
Product Name Price (net) Qty
Cat. No.: A 019-06
Unit: 0.6 ml
$162.00
Cat. No.: A 019-25
Unit: 2.5 ml
$506.00
Cat. No.: A 019-60
Unit: 6 ml
$1,019.00
The second messenger cyclic GMP immobilized on agarose by an aminoethylthio spacer attached to position 8 of the ligand. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This type of gel is offered as PDE-resistant version (Sp-8-AET-cGMPS-Agarose, Cat. No. A 010) as well. Detailed technical information available. References: Schlossmann et al., Nature, 404, 197 - 201 (2000); Scholten et al., J. Proteome Res., 5, 1435 - 1447 (2006); Antl et al., Blood, 109, 552 - 559 (2007); Margarucci et al., Mol. BioSyst., 7, 2311 - 2319 (2011).
Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
Cat. No. A 019
CAS number [pending]
Storage temperature 4°C / 39°F
Modifications 8-cGMP
  • 1. Donaldson L.Meier S., Methods Mol. Biol., 1016, 155 - 173 (2013), "An Affinity Pull-Down Approach to Identify the Plant Cyclic Nucleotide Interactome"
  • 2. Margarucci L., Roest M., Preisinger C., Bleijerveld O. B., van Holten T. C., Heck A. J. R.Scholten A., Mol. Biosyst., 7, 2311 - 2319 (2011), "Collagen Stimulation of Platelets Induces a Rapid Spatial Response of cAMP and cGMP Signaling Scaffolds"
  • 3. Antl M., von Brühl M.-L., Eiglsperger C., Werner M., Konrad I., Kocher T., Wilm M., Hofmann F., Massberg S.Schlossmann J., Blood, 109, 552 - 559 (2007), "IRAG Mediates NO/cGMP-dependent Inhibition of Platelet Aggregation and Thrombus Formation"
  • 4. Scholten A., Poh M. K., Vandekerckhove J., van Breukelen B., Vos M. A.Heck A. J. R., J. Proteome Res., 5, 1435 - 1447 (2006), "Analysis of the cGMP/cAMP Interactome Using a Chemical Proteomics Approach in Mammalian Heart Tissue Validates Sphingosine Kinase Type 1-interacting Protein as a Genuine and Highly Abundant AKAP"
  • 5. Diaz C. A., Alloco J., Powles M. A., Yeung L., Donald R. G. K., Anderson J. W.Liberator P. A., Mol. Biochem. Parasitol., 146, 78 - 88 (2006), "Characterization of Plasmodium falciparum cGMP-Dependent Protein Kinase(PfPKG): Antiparasitic Activity of a PKG Inhibitor"
  • 6. Kim E.Park J. M., J. Biochem. Mol. Biol., 36, 299 - 304 (2003), "Identification of Novel Target Proteins of Cyclic GMP Signaling Pathways Using Chemical Proteomics"
  • 7. Gurnett A. M., Liberator P. A., Dulski P. M., Salowe S. P., Donald R. G. K., Anderson J. W., Wiltsie J., Diaz C. A., Harris G., Chang B., Darkin-Rattray S. J., Nare B., Crumley T., Blum P. S., Misura A. S., Tamas T., Sardana M. K., Yuan J., Biftu T.Schmat, J. Biol. Chem., 277, 15913 - 15922 (2002), "Purification and Molecular Characterization of cGMP Dependent Protein Kinase from Apicomplexan Parasites: A Novel Chemotherapeutic Target"
  • 8. Schlossmann J., Ammendola A., Ashman K., Zong X., Huber A., Neubauer G., Wang G.-X., Allescher H.-D., Korth M., Wilm M., Hofmann F.Ruth P., Nature, 404, 197 - 201 (2000), "Regulation of Intracellular Calcium by a Signalling Complex of IRAG, IP3 Receptor and cGMP Kinase I(Beta)"
  • 9. Boadu E.Sager G., Naunyn-Schmied. Arch. Pharmacol. XIIIth. Int. Congr. Pharmacol. Munich R 413 P 3.19, 358, 2 - 0 (1998), "Characterization of the Transporter Responsible for Cellular cGMP Export"