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Cyclic AMP connected to the relatively small Pharos 575 dye (λexc 577 nm, λem 605 nm) by a spacer. The fluorophore can be excited e.g. by a Kr/Ar laser. Due to its high lipophilicity and bright red fluorescence 8-[φ-575]-cAMP is especially suitable for studies with intact cells. Upon binding to PKA a small hypsochromic shift is observed. Detailed technical information available. Reference: Moll et al., BMC Biochem., 9: 18 (2008).
|Cat. No.||P 019|
|Purity||> 98% HPLC|
|Storage temperature||-20°C / -4°F|
|Target||PKA (cAK) Activator|
|Excitation max [nm]||577|
|Emission max [nm]||605|
|Absorption max [nm]||575|
|Molar extinction coefficient ε [L·mol⁻¹·cm⁻¹]||20960|